Bruker Daltonik Gmbh

Other

Ulrich Weller

Markus Kostrzewa

Katrin Sparbier

The invention relates to the detection of specified, flagellated bacteria, particularly, in food and stool. A single culturing period of about 12 to 24 hours in a liquid nutrient medium without agitation is combined with a position-selective sampling of the flagellated microbes from the liquid of the culture, after which a mass spectrometric detection method is used which recognizes the target bacteria in mixtures. A second culture step is only necessary in exceptional cases. A species-selective or genus-selective culture medium is advantageous. Positional selection becomes possible because these bacteria use their flagella to counteract sedimentation by chemotaxis, and they collect near the surface. This provides a low-cost detection method that is several days faster than conventional methods

[object Object]

The invention concerns the reduction of the risk of an incorrect assignment of samples to sample sites during the manual deposition of samples for ionization by laser desorption (for example MALDI or LDCI). The invention offers a method wherein a sample support with several sample sites is provided, at least one sample site is selected, and the selected sample site is highlighted, at least in contrast to neighboring not selected sample sites, in a way which the human eye can perceive.

Deposition aid for the manual deposition of mass spectrometric samples

Identification of infectious pathogens, particularly viruses, bacteria and other microorganisms is effected with a method whereby pathogens of acute infections can be identified, without first culturing them in external nutrient media, by mass spectrometric measurement of their protein profiles obtained from pathogens directly precipitated from body fluid into pellets by centrifuging. With this method, pathogens which cause acute infections can be identified in less than one hour.

Growing community of inventors

Cologne, Germany

Ulrich Weller