Roche Sequencing Solutions, Inc.

Leland Stanford Junior University

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Ulrich Schlecht

Ronald W Davis

Yann A Astier

Jan Berka

Janine Mok

Frantisek Foret

Ivona Voracova

Joseph L Horecka

Colin Harvey

Robert P St Onge

Vladimira Datinska

Sasha F Levy

Jenny A Johnson

Austin So

Maureen Elizabeth Hillenmeyer

Michael George

Duylinh Nguyen

Yann Astier

Jaeyoung Yang

The present disclosure generally relates to devices and methods for effecting epitachophoresis. Epitachophoresis may be used to effect sample analysis, such as by selective separation, detection, extraction, and/or pre-concentration of target analytes such as, for example, DNA, RNA, and/or other biological molecules. Said target analytes may be collected following epitachophoresis and used for desired downstream applications and further analysis.

Devices for sample analysis using epitachophoresis

The invention comprises circular single-stranded templates for nucleic acid sequencing, methods of making and using the same.

Method for generating single-stranded circular DNA libraries for single molecule sequencing

Inducible promoters for the coordinated expression of at least one heterologous gene in yeast and methods of using them are disclosed. In particular, the invention relates to sets of inducible promoters derived fromand related species that can be induced in the presence of nonfermentable carbon sources.

Inducible production-phase promoters for coordinated heterologous expression in yeast

The present disclosure generally relates to devices for effecting epitachophoresis. Epitachophoresis may be used to effect sample analysis, such as by selective separation, detection, extraction, and/or pre-concentration of target analytes such as, for example, DNA, RNA, and/or other biological molecules. Said target analytes may be collected following epitachophoresis and used for desired downstream applications and further analysis.

A novel method for preparing sequence-verified oligonucleotides is disclosed. In particular, the invention relates to a simple, affordable, and scalable method that combines high-throughput mating of yeast clones, a unique selectable system for combining DNA sequences in yeast, and next-generation sequencing. This method allows sequence-verified oligonucleotides to be readily isolated from complex libraries.

Scalable method for isolation and sequence-verification of oligonucleotides from complex libraries

The invention is a novel method of making and using a library such as a sequencing library of single stranded circular nucleic acid templates via splint ligation.

Circularization methods for single molecule sequencing sample preparation

Growing community of inventors

Sunnyvale, CA, United States of America

Ulrich Schlecht