Boehringer Mannheim Gmbh

Ulrich Mayr

Hans Seidel

Joachim Siedel

Hans Mollering

Helmgard Gauhl

The present invention provides a process for obtaining sarcosine oxidase from micro-organisms by culturing thereof and obtaining the enzyme from the biomass or from the culture broth, wherein a Pseudomonas strain is cultured.

Process for obtaining sarcosine oxidase from microorganisms

The present invention provides a method of use for a hydrogen peroxide-forming oxidase, wherein the enzyme is obtained from Streptomycetaceae and at 25.degree. C., in 0.15 mol/liter potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40% of the initial activity. This enzyme is useful in the determination of sarcosine, creatine and creatinine.

Method for the determination of sarcosine creatine or creatinine

The present invention provides a hydrogen peroxide-forming sarcosine oxidase, wherein it is obtainable from Streptomycetaceae and at 25.degree. C. in 0.15 mol/liter potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40% of the initial activity.

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Rosenheim, Germany

Ulrich Mayr