Behringwerke Aktiengesellschaft

Aventis Behring Gmbh

Zlb Behring Gmbh

Ulrich Grundmann

Egon Amann

Gerd Zettlmeissl

Karl-Josef Abel

Eugen Amann

Gerhard Dickneite

Hubert Metzner

Richard Lathe

Meng Li

Austin Smith

Gerd Zettlmeibl

The cDNA which codes for factor XIIIa has been isolated using a cDNA bank from human placenta and probes constructed on the basis of the amino acid sequence of factor XIIIa peptide fragments. It is possible with this cDNA not only to obtain factor XIIIa by gene manipulation in high purity but also to prepare diagnostic aids which permit the analysis of genetic factor XIIIa defects. Furthermore, it is possible on the basis of the amino acid sequence to prepare antibodies which are suitable for diagnostic aids and antibody columns.

Preparation of factor XIIIa by gene manipulation

Preparation of DNA encoding factor XIIIA

The invention provides a transgenic mouse which is heterozygous or homozygous for an at least partially defective coagulation factor XIII gene.

Transgenic coagulation factor XIII defective animal and its use for testing wound healing and bleeding

The protein PP4-X, whose amino acid sequence and the DNA sequence coding for this have been determined, has anticoagulative properties and can be prepared by genetic manipulation. PP4-X is used for therapeutic and diagnostic purposes.

DNA encoding anticoagulative protein PP4-X, and its preparation and use

Specific fragments of the protein PP15 were prepared by enzyme cleavage. Using statistical data, specific oligonucleotides were selected from the oligonucleotides coding for the specific PP15 fragments. These oligonucleotides were then used as probes to successfully screen a cDNA bank of mature human placenta and isolate the complete cDNA of PP15. The cDNA was sequenced and characterized. This cDNA can be used to prepare PP15 protein in prokaryotic or eukaryotic cells.

Protein PP 15 preprared by genetic manipulation

Anticoagulative protein PP4-X

The cDNA coding for PP15 is described. This cDNA can be used to prepare PP15 in pro- or eukaryotic cells.

Protein PP15 prepared by genetic manipulation

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Marburg, Germany

Ulrich Grundmann