Other

Ulf-Hangkan Stenman

Hans Lilja

Johan Hedstrom

Assay of free and complexed trypsinogen-2

According to the method of the invention, immunoassays are applied to measure free PSA as well as a proteinase inhibitor complex. Free PSA and PSA complex are according to the invention measured by a non-competitive immunoassay employing at least two different monoclonal antibodies. The invention is further characterized by that the PSA proteinase inhibitor complex of interest is formed either with .alpha..sub.1 -antichymotrypsin, .alpha..sub.1 -protease inhibitor (API) or .alpha..sub.2 -macroglobulin. Moreover, the invention is characterized by that free PSA, the PSA-proteinase inhibitor complex and their ratio are applied in the diagnosis of patients with prostate cancer.

Assay of free and complexed prostate-specific antigen (PSA)

Prostate specific antigen (PSA) is a protein that can form complexes with proteinase inhibitors such as .alpha..sub.1 -antichymotrypsin. The invention is characterized in that the measurement of the ratio of free PSA to PSA bound to proteinase inhibitors (free PSA:PSA-proteinase inhibitor complex) is useful in the diagnosis of patients with prostate cancer.

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Grankulla, Finland

Ulf-Hangkan Stenman