Biotest Pharma Gmbh

Association Pour L'essor De La Transfusion Sanguine Dans La Region Du

Ulf Bethke

Michael Kloft

Dominique Bourel

Roland Beliard

Dieter Rudnick

Wolfgang Moller

Norbert Kothe

Detlef Rohm

Magali Holuigue

Peter Byrne

Herve Broly

Ahmed Bouzidi

The invention relates to 2 human monoclonal antibodies of sub-classes IgG1 and IgG3, against the Rhesus D antigen and to a pharmaceutical composition containing a mixture of the said antibodies, more particularly intended for the prophylaxis of the haemolytic disease of the newborn. The invention also relates to the heterohybridoma cell lines that produce these antibodies, which are filed with the 'Deutsche Sammlung von Mikroorganismen und Zellkulturen' under accession numbers DSM AC 2039 and DSM AC 2040.

Human monoclonal anti-rhesus (D) antibodies and cell lines producing same

To provide a simple method of preparing biologically active transferrin that can be employed on an industrial scale and that will result in extremely pure and natural transferrin containing no viruses and appropriate for both in-vitro and in-vivo applications, the .gamma.-globulins are precipitated from the fraction containing the transferrin, the precipitant is removed from the residual liquid by ultrafiltration or gel filtration, the liquid is adjusted to a prescribed ionic concentration and protein concentration and (a) treated with .beta.-propiolactone and the solution is subjected to ultraviolet radiation or (b) treated with specific detergents and subjected to ion-exchange chromatography, and the transferrin is concentrated and filtered sterile.

Method of preparing a high-purity, virus safe, biologically active

Growing community of inventors

Babenhausen, Germany

Ulf Bethke