Altria Client Services LLC

Micreos B.v.

Ujwala Warek

Dongmei Xu

James A Strickland

Elisabeth Miller

Yanxin Shen

Chengalrayan Kudithipudi

Jesse Frederick

Steven Hagens

Mark T Nielsen

Jaemo Yang

Mingwu Cui

Yahya Karga

James Powell

Xue Luo

Xiaohong Jin

Kwang Choi

Dong Qi

Fritz Eichenseher

John Kotrola

A stabilizing process for tobacco and tobacco products includes sterilizing the tobacco by applying an electron beam treatment to the tobacco. The process may also include treating the tobacco. The treating the tobacco includes pre-conditioning the tobacco, pasteurizing the tobacco, curing the tobacco, fermenting the tobacco, or combinations thereof. The method may also include forming a smokeless tobacco product containing the tobacco.

Stabilization methods for tobacco and tobacco products

The present disclosure provides the identification of promoters that are preferentially active in tobacco axillary buds. Also provided are tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing tobacco plants comprising reduced or no sucker growth.

Optimized tissue-preferred promoter and uses thereof

This disclosure provides a number of sequences involved in the transport of alkaloids from the root to the leaf in tobacco, methods of using such sequences, tobacco plants carrying modifications to such sequences, and tobacco products made from such plants.

Tobacco plants having altered amounts of one or more alkaloids in leaf and methods of using such plants

The present disclosure provides compositions and methods related to tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

Compositions and methods based on PMT engineering for producing tobacco plants and products having altered alkaloid levels

Bacteriophage againstare provided, and methods of making and using the bacteriophage also are provided.

Endolysin from bacteriophage against geobacillus and methods of using

Bacteriophage are provided, and methods of making and using the bacteriophage also are provided.

Bacteriophage and methods of making and using

Provided herein are bacterial strains that are capable of degrading nitrite and/or TSNAs. Also provided herein are methods of using such bacterial strains to degrade nitrite and/or TSNAs.

Nitrite-degrading and TSNA-degrading bacteria and methods of making and using

Bacteriophage are provided, and methods of making and using the bacteriophage also are provided. The bacteriophage can be provided in a tobacco product, where the bacteriophage reduces the number of viable bacteria in the tobacco product and includes a nucleic acid sequence encoding an endolysin having at least 95% sequence identity to the nucleic acid sequence shown in SEQ ID NO: 1 or to the amino acid sequence shown in SEQ ID NO: 2.

Endolysin from bacteriophage againstand methods of using

Methods of making reconstituted tobacco include contacting a mixture including tobacco pulp with one or more fluid streams including one or more peroxides (e.g., hydrogen peroxide), recovering one or more fluids from the tobacco pulp mixture, and reusing the recovered fluids in at least one subsequent tobacco pulp contacting step. In some cases, methods provided herein can recover one or more fluids including one or more peroxides, such that the at least one subsequent tobacco pulp contacting step includes contacting tobacco pulp with a fluid including one or more peroxides.

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Chester, VA, United States of America

Ujwala Warek