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Leibniz-institut Für Pflanzengenetik Und Kulturpflanzenforschung (ipk)

Novoplant Gmbh

Leibniz-institut Fur Pflanzengenetik Und Kulturpflanzenforschung

Udo Conrad

Sergej Kiprijanov

Jürgen Scheller

Mario Gils

Olga Artsaenko

Hoang Phan

Ulrike Fielder

Doreen Jahn

Thuong Thi Ho

Julian Phillips

Valeska Hauptmann

Hoang Trong Phan

Martin Giersberg

Sigrun Hähnel

Karl-Heinz Guehrs

Frank Grosse

The present invention relates to a method for production of an oligomeric protein in eukaryotic cells by co-expression of two fusion proteins in eukaryotic cells comprising a protein-S-Tag fusion protein, wherein the protein is an antigen or an antibody, and a S-protein-tail piece (tp) fusion protein. Furthermore the present invention relates to an oligomeric protein comprising at least a protein-S-Tag fusion protein and a S-protein-tail piece (tp) fusion protein, wherein the protein of the protein-S-Tag fusion protein is an antigen or an antibody, and the use in vaccines.

Oligomeric vaccines from plants by S-Tag-S-protein fusions

The invention relates to cassettes for the expression of storable gene products in leaves and specifically in seeds, especially single-chain antibody fragments in leaves and seeds of transgenic tobacco and pea plants. The fields of application of the invention are biotechnology, medicine (diagnosis and therapy), foodstuffs and plant control and agriculture. The expression cassette of the invention comprises constitutive or seed-specific promoters, the LeB4 signal peptide, a gene to be expressed and an ER retention signal. Preference is given to an expression cassette containing the CaMV 35S promoter as the constitutive promoter, the gene for a single-chain antibody fragment as the gene and the amino acid sequence KDEL as the ER retention signal.

Cassettes for the expression of storable proteins in plants

Growing community of inventors

Munich, Georgia

Udo Conrad