Liqbio Biomedical Company Limited

Ace Medical Technology Co., Ltd.

Chang Gung University

Tzu-Keng Chiu

Cheng-Fang Yang

Yu-Xian Zhu

Chi-Kun Ohyang

Min-Hsien Wu

Wen-Pin Chou

Chia-Hsun Hsieh

Po-Yu Chu

Chi-Kun Oh Yang

Hung-Ming Wang

Huan-Yi Tseng

Yu-xian Zhu

Cell flow channel

A detecting device for detecting biological particles includes an optical system including an excitation light source, a filter and spectroscope group, a photomultiplier tube, and a charge-coupled device. The excitation light source illuminates the biological particles on a detecting carrier of the detecting device. A kind of target biological particles in the biological particles is excited to generate an emission light. The emission light enters the filter and spectroscope group to be separated into a first detecting light and a second detecting light. After the photomultiplier tube receives the first detecting light, the photomultiplier tube transmits a regional positioning signal to a processor of the detecting device. After the charge-coupled device receives the second detecting light, the charge-coupled device transmits an image signal to the processor. The processor obtains a precise location of the target biological particles based on the regional positioning signal and the image signal. A detecting method of the detecting device is also provided.

Detecting device for detecting biological particles and detecting method thereof

Biological particle detecting tray

A sensing method of bioparticle positioning includes the steps of: providing a carrier divided into multiple detection areas; adding bioparticle sample in the carrier, wherein the bioparticle sample includes first bioparticle with biomarker and interacts with corresponding tag; providing excitation energy that makes the tag on the first bioparticle emit radioactive energy; moving the first sensor to the detection area respectively; after receiving radioactive energy, defining the detection area where the radioactive energy comes from as activity detection area, and sending location information of the activity detection area to processing module; according to location information, moving second sensor to detection area, detecting the accurate location of the first bioparticle in activity detection area, and sending the accurate location to processing module. A bioparticle positioning sensing system is also provided herein. The method and system above detect specific bioparticle quickly and improve the detection efficiency.

Sensing method of bioparticle positioning and bioparticle positioning sensing system

A device for sorting bio-particles by image-manipulated electric force includes a first substrate, a second substrate, a fluidic channel, one or more photosensitive layers and an inlet hole. The first substrate has a first conductive electrode, and the second substrate has a second conductive electrode. The second conductive electrode is disposed opposite the first conductive electrode. The fluidic channel is disposed between the first conductive electrode and the second conductive electrode. The photosensitive layer is conformally disposed on at least one of the surfaces of the first conductive electrode and the second conductive electrode. The inlet hole is disposed in the first conductive electrode and the first substrate, where the inlet hole includes a first opening close to the fluidic channel and a second opening away from the fluidic channel, and the surface area of the first opening is greater than the surface area of the second opening.

Device for sorting bio-particles using image-manipulated electric force and operating method thereof

A method of screening, isolating and purifying rare cells includes obtaining cell groups with rare cells from a sample, removing cells not to be screened from the cell groups, and drawing the remaining cells as a cell suspension sample. An introduction device is activated to load the cell suspension sample onto an optically-induced dielectrophoresis chip including a cell isolation zone having a division arrangement wherein at least one optical pattern is configured on the cell isolation zone for screening, isolating and purifying the rare cells. The at least one optical pattern is configured to migrate, rotate or both. The at least one optical pattern is activated to attract the rare cells into a collection tank on the chip, and a liquid drawing device is activated to draw the rare cells out of the collection tank, thereby obtaining purified rare cells from the sample.

Method of screening, isolating, and purifying rare cells

A method for performing contactless ODEP for separation of CTCs is provided with the steps of obtaining patients' blood with rare cell suspected CTCs; adding at least one fluorescent antibody binding to CTCs into the blood; staining the blood; injecting the stained blood with fluorescent dye into an ODEP device and then performing fluorescent image identification; trapping the CTCs with at least one fluorescent antibody in the ODEP device by creating an image pattern and then generating an ODEP force; Separating the trapped CTCs from other non-CTCs cells; absorbing the trapped CTCs; and obtaining a high purity of CTCs. An apparatus for performing contactless ODEP for separation of CTCs is also provided.

Method and apparatus for performing contactless optically-induced dielectrophoresis for separation of circulating tumor cells

Growing community of inventors

Taipei, Taiwan

Tzu-Keng Chiu