Chung Yuan Christian University

Council of Agriculture, Executive Yuan

National Defensive Medical Center

Tzong-Yuan Wu

Ying-Ju Chen

Suey-Sheng Kao

Chang-Chi Lin

Szu-Cheng Kuo

Tzyy-Rong Jinn

Mean-Shine Chen

Chao-Yi Teng

Tsu-An Hsu

Shin-Ru Shih

Yi-Ting Lin

Chien-Ling Su

Shu-Fen Chang

Shin-Jhan Zeng

Der-Jiang Chiao

Pei-Yun Shu

Yen-Kuang Li

Yui-Whei Chen-Yang

Ching-Yao Yuan

Ming-Kun Liu

Yu-Ming Wang

Chung-Hsiung Wang

Disclosed herein are recombinant baculoviruses suitable for detecting the presence of arthropod-borne viruses in a biological sample of a test subject. The information derived from the detection may also be used to render a diagnosis on whether the test subject is infected with the arthropod-borne viruses or not, so that proper course of treatment may be assigned to the subject.

Method of detecting antibodies against JEV or ZIKV using recombinant baculoviruses expressing prM/e

Recombinant baculoviruses and their uses in detecting arthropod-borne virus

Disclosed herein is a method for the treatment of an infection with, or disease caused by, Chikungunya virus in a subject. The method includes administering to the subject a therapeutically effective amount of suramin as the active agent.

Method for treating Chikungunya virus infection

Disclosed herein is a recombinant viral construct and its uses thereof. The recombinant viral construct is capable of simultaneously expressing three exogenous proteins, including a classical swine fever virus (CSFV) antigen, a porcinetype 2 (PCV2) antigen, and an immunomodulatory protein. The recombinant viral construct is hence useful as a bio-tool for simultaneously producing multiple antigens of a bi-subunit vaccine.

Recombinant baculovirus vector and uses thereof

Disclosed herein is anmultiple nucleopolyhedrovirus (AcMNPV) based hybrid baculovirus and its uses thereof. The AcMNPV based hybrid baculovirus is capable of infecting different hosts, and comprisesmultiple nucleopolyhedrovirus (MaviMNPV) genes of lef1, orf1629, pk1, CDS1, CDS2, and lef2; and AcMNPV/MaviMNPV-hybrid genes of egt and orf152. The AcMNPV based hybrid baculovirus is therefore useful as a bio-insecticide by its capability of delivering genes of toxic proteins to be expressed in at least two different insect hosts.

Hybrid baculovirus and uses thereof

Disclosed herein is anmultiple nucleopolyhedrovirus (AcMNPV) based hybrid baculovirus and its uses thereof. The AcMNPV based hybrid baculovirus is capable of infecting different hosts, and comprisesnuclear polyhedrosis virus (BmNPV) genes of lef9, vlf1, p6.9, and vp80;multiple nucleopolyhedrovirus (MaviMNPV) genes of lef2, orf1629, and pe38; and AcMNPV/BmNPV/MaviMNPV-hybrid genes of pk1, lef8, DNA pol, GP41, helicase, orf94, VP1054, orf65, lef4, lef5, and orf99. The AcMNPV based hybrid baculovirus is therefore useful as a bio-tool or bio-insecticide for its capability of delivering genes for production or expression of toxic proteins in at least three different insect hosts.

Hybrid baculovirus having the capability of infecting at least three insect hosts and uses thereof

The present invention discloses a method for fabricating aerogels, a method for fabricating surface-modified aerogels, and a method for fabricating biocomposites. Take the fabricating method of biocomposites for example, first, a precursor solution is provided and the precursor solution comprises a hydrophilic ionic liquid, a catalyzed hydrolysis and/or condensation reagent, at least one biomolecule. Next, a curing process is performed for the precursor solution to hydrolyze and polymerize the at least one alkoxide monomer and/or aryloxide monomer to wrap at least one biomolecule and thus form biocomposite. Afterwards, an extracting process is performed by a solvent for the biocomposite to substitute the ionic liquid in the biocomposite. Finally, a drying process for the biocomposite is carried out after the extracting process so as to remove the solvent in the biocomposite. Therefore, the biocomposite is formed.

Method for fabricating a biocomposite comprising an aerogel wrapping a biomolecule

An improved baculovirus vector capable of expressing genes in mammalian or insect host cells, and the uses thereof are disclosed. The improved baculovirus vector includes in sequence: a promoter; a first nucleic acid operably linked to the promoter for expressing a first protein in the mammalian or insect host cells; a chimera internal ribosomal entry site (IRES) comprising a portion of an enterovirus (EV) IRES sequence at least 90% identical to SEQ ID NO: 1 and a portion of avirus (RhPV) IRES sequence at least 90% identical to SEQ ID NO: 2; and a second nucleic acid operable linked to the portion of the RhPV IRES sequence for expressing a second protein in the mammalian or insect host cells.

Chimeral internal ribosomal entry site sequence and uses thereof

The present invention relates to use of bicistronic DNA constructs for identifying compounds that inhibits IRES-dependent translation activity of an infectious enterovirus (EV) or encephalomyocarditis virus (EMCV) without affecting CAP-dependent translation activity of a host subject. The compounds thus identified are useful in preparation of a medicament for treating EV or EMCV infection.

Use of biocistronic DNA constructs for identifying compounds that inhibit IRES-dependent translation

An insect larva aerosol infection method for producing recombinant proteins and baculovirus bio-insecticides is disclosed. A liquid spray of budded form baculoviruses are employed to infect insect larvae in order to reduce the manpower for manual injections and the energy wasted in feeding infection technologies. The invention may be applied to the production of recombinant proteins and baculoviruses as the production platform for bio-insecticides.

Insect larva aerosol infection method for producing recombinant proteins and baculovirus bio-insecticides

Use of biscistronic DNA constructs for identifying compounds that inhibit IRES-dependent translation

Growing community of inventors

Panchiao, Taiwan

Tzong-Yuan Wu