Kaohsiung Medical University

Tung-Ying Wu

Yang-Chang Wu

Fang-Rong Chang

Ying-Chi Du

Mei-Chin Lu

Yu-Ming Hsu

Disclosed are a method for analyzing chemical profiles of components from a herbal medicine product. Components may include quinone (including rhein, sennoside A and/or aloe-emodin), stilbene containing resveratroloside, flavone including baicalin, and/or alkaloid including berberine and/or palmatine. The method includes steps of: (a) respectively chromatographing a methanol extract of product and standard(s) corresponding to the component(s) using HPLC; (b) comparing HPLC chromatogram of extract and standard(s); and (c) analyzing the chemical profiles of the product from the comparison results.

Chemical profile of detecting bioactive components of quinones, stilbenes, flavones and alkaloids

A preparation method for a water extract of the fruiting body of(ACW) is provided. The method includes steps of: (a) providing the fruiting body; and (b) boiling the fruiting body in water to obtain the water extract. This polysaccharide-rich water extract frominduces the maturation of dendritic cells, enhances T cell proliferation and INF-&#x3b3; production, and polarizes them toward the Th1 pathway. ACW can be effectively applied in cancer immunotherapy.

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A preparation method for an ethanol extract of the fruiting body of(EEAC) is provided. The preparation method includes steps of: (a) providing the fruiting body of(AC); (b) extracting the fruiting bodies with a first ethanol solution; and (c) obtaining EEAC. EEAC further can be sequentially extracted or fractioned by n-hexane, ethyl acetate and ethanol, and an n-hexane fraction (FC), an ethyl acetate fraction (FA) and an ethanol fraction (FB) respectively are generated. The growth inhibition and apoptosis induction of leukemia cell line HL 60 are effectively mediated by FA product, in which zhankuic acid A is the bioactive marker. The amount of triterpenoid in the fruiting body of AC can be determined by NMR and HPLC analysis.

Disclosed are a method for preparing an n-hexane extract of the fruiting body of(AC), wherein the fruiting body of AC is sequentially extracted with the ethanol solution and the n-hexane solution to obtain the n-hexane extract containing at least one benzenoid compound. The amounts of 4,7-dimethoxy-5-(3-methylbut-3-en-1-ynyl)-6-methyl-1,3-benzodioxole, 4,7-dimethoxy-5-methyl-1,3-benzodioxole, antrocamphine A and the combination thereof in the at least one benzenoid compound are determined using chromatography, NMR and HPLC. In addition, the present invention is applicable on detecting the amounts of benzenoid compounds in the AC healthcare food/drug or the fruiting body of AC, and thus owns the industrial values.

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Kaohsiung, Taiwan

Tung-Ying Wu