Beckman Coulter, Inc.

Tung-Liang Huang

M Parameswara Reddy

Chitra Kumari Ratnayake

Daniel A Keys

An article of manufacture is provided that is useful in differentiating between solutes, such as during electrophoretic separations. An embodiment of the article is a capillary tube, that carries a polymer along the inner wall of the capillary tube. The polymer is effective to reduce undesired interactions and preferably includes a polylactam that is absorbed to the surface before the surface is exposed to the solutes. A preferred polylactam is poly(vinylpyrrolidone) with a molecular weight greater than about 1,000,000 daltons (weight-average).

Surfaces with reduced electroosmotic flow

Reagents and methods for preparing samples containing both biomolecule analytes and macrobiomolecules for capillary electrophoresis separation are provided. The reagents comprise a branched polymer. When mixed with a sample containing both biomolecule analytes and macrobiomolecules, particularly DNA templates, the branched polymer of the reagent can suppress the entrance of the macrobiomolecules into a capillary electrophoresis tube during electrokinetic injection of the sample into the capillary electrophoresis tube.

Method and reagent for suppressing the injection of nucleic acid sequencing template into micro-bore capillary during DNA separation with capillary electrophoresis

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Placentia, CA, United States of America

Tung-Liang Huang