The United States of America As Represented by the United States

University of California

Other

US Government As Represented by the Secretary of the Army

Hinds Instruments, Inc.

Cell Robotics, Inc.

Tudor N Buican

John C Martin

Arthur H Carrieri

Erik S Roese

Alan C Samuels

James J Sutter

Thomas M Yoshida

Bryan D Upham

Kevin M Carlson

A pseudo-active chemical imaging sensor including irradiative transient heating, temperature nonequilibrium thermal luminescence spectroscopy, differential hyperspectral imaging, and artificial neural network technologies integrated together. The sensor may be applied to the terrestrial chemical contamination problem, where the interstitial contaminant compounds of detection interest (analytes) comprise liquid chemical warfare agents, their various derivative condensed phase compounds, and other material of a life-threatening nature. The sensor measures and processes a dynamic pattern of absorptive-emissive middle infrared molecular signature spectra of subject analytes to perform its chemical imaging and standoff detection functions successfully.

Sensor system and method for the panoramic standoff detection of chemical contaminants on terrestrial surfaces

A photoelastic modulator excited by a plurality of piezoelectric transducers affixed to the surface of a photoelastic bar for generating elastic waves in a plurality of cells each bounded by piezoelectric node drivers between which an antinode driver is disposed, is described. The standing elastic wave in each cell can be made independent of those in the neighboring cells by controlling the phase and amplitude of the drivers. The resulting elastic waves generate birefringence along the optical axis of the bar such that a linearly polarized optical wave propagating along the axis and having an initial phase, experiences a retardation of the phase as it passes through each cell. The cells may be controlled such that the phase of each standing wave does not change between cells and the phase retardation of the optical wave increases monotonically as the optical wave propagates through the bar, thereby, in theory, permitting an arbitrarily large retardation to be generated.

High retardation-amplitude photoelastic modulator

A system and method for exploiting the dependance of a photoelastic modulator's (PEM's) resonance frequency on temperature (attributable to driving amplitude and to ambient temperature) to generally improve the performance of PEMs. In one embodiment there is provided a method and system for efficiently driving a series of multiple PEMs. To this end, each of the PEMs in a stack are separately tuned, as by controlling the power dissipated in each PEM, so that the resonance frequencies of all of the PEMs converge to a common frequency. Thus, all of the PEMs are simultaneously at resonance to ensure maximum efficiency and to maintain a selected total retardation amplitude. In another embodiment of the present invention, a single-element PEM is controlled in a manner to account for the subtle changes in the PEM's resonance frequency. To this end a control method and system is provided to keep constant both the retardation amplitude of the PEM and the actual operating frequency of the PEM, which frequency need not necessarily be the resonant frequency.

Controlling resonant photoelastic modulators

A chamber is disclosed, as for example an optical manipulation chamber, which has a pair of spaced windows and a central core member between the windows. The windows are aligned in parallel planes and the central core member contains a plurality of compartments and channels. A radiation curable resin is used, in combination with injection ports, to form the channels and compartments. The configuration of the channels and compartments is placed on a mask having shielding and nonshielding regions. Radiation is passed through the mask as the resin is injected through the ports, thereby curing the resin which is exposed to the radiation. The channels and compartments can be accurately designed in minute detail.

Method for the manufacture of an optical manipulation chamber

Apparatus and method for measuring intensities at a plurality of wavelengths and lifetimes. A source of multiple-wavelength electromagnetic radiation is passed through a first interferometer modulated at a first frequency, the output thereof being directed into a sample to be investigated. The light emitted from the sample as a result of the interaction thereof with the excitation radiation is directed into a second interferometer modulated at a second frequency, and the output detected and analyzed. In this manner excitation, emission, and lifetime information may be obtained for a multiplicity of fluorochomes in the sample.

Apparatus and method for measuring fluorescence intensities at a

An integrated fluorescence analysis system enables a component part of a sample to be virtually sorted within a sample volume after a spectrum of the component part has been identified from a fluorescence spectrum of the entire sample in a flow cytometer. Birefringent optics enables the entire spectrum to be resolved into a set of numbers representing the intensity of spectral components of the spectrum. One or more spectral components are selected to program a scanning laser microscope, preferably a confocal microscope, whereby the spectrum from individual pixels or voxels in the sample can be compared. Individual pixels or voxels containing the selected spectral components are identified and an image may be formed to show the morphology of the sample with respect to only those components having the selected spectral components. There is no need for any physical sorting of the sample components to obtain the morphological information.

Integrated fluorescence analysis system

A particle control chamber enables experiments to be carried out on biological cells and the like using a laser system to trap and manipulate the particles. A manipulation chamber provides a plurality of inlet and outlet ports for the particles and for fluids used to control or to contact the particles. A central manipulation area is optically accessible by the laser and includes first enlarged volumes for containing a selected number of particles for experimentation. A number of first enlarged volumes are connected by flow channels through second enlarged volumes. The second enlarged volumes act as bubble valves for controlling the interconnections between the first enlarged volumes. Electrode surfaces may be applied above the first enlarged volumes to enable experimentation using the application of electric fields within the first enlarged volumes. A variety of chemical and environmental conditions may be established within individual first enlarged volumes to enable experimental conditions for small scale cellular interactions.

Chamber for the optical manipulation of microscopic particles

An apparatus and method simultaneously measures a plurality of spectral wavelengths present in electromagnetic radiation. A modulatable birefringent optical element is employed to divide a polarized light beam into two components, thereby producing a phase difference in two resulting light beams such that the two beams can be made to interfere with one another when recombined, the interference pattern providing the wavelength information required for the analysis of the incident light. The interferometer thus created performs in a similar manner to a Michelson interferometer, but with no moving parts, and with a resolution dependent on the degree of phase shift introduced by the modulator.

Method and apparatus for simultaneously measuring a plurality of

Method and apparatus for sorting particles, such as biological particles. A first laser defines an optical path having an intensity gradient which is effective to propel the particles along the path but which is sufficiently weak that the particles are not trapped in an axial direction. A probe laser beam interrogates the particles to identify predetermined phenotypical characteristics of the particles. A second laser beam intersects the driving first laser beam, wherein the second laser beam is activated by an output signal indicative of a predetermined characteristic. The second laser beam is switchable between a first intensity and a second intensity, where the first intensity is effective to displace selected particles from the driving laser beam and the second intensity is effective to propel selected particles along the deflection laser beam. The selected particles may then be propelled by the deflection beam to a location effective for further analysis.

Growing community of inventors

Albuquerque, NM, United States of America

Tudor N Buican