The Chinese University of Hong Kong

Grail, Inc.

Tsz Kwan Sin

Kwan Chee Chan

Rossa Wai Kwun Chiu

Yuk-Ming Dennis Lo

Peiyong Jiang

Lu Ji

Haiqiang Zhang

Jiaen Deng

Techniques are provided for analyzing circular DNA in a biological sample (e.g., including cell-free DNA, such as plasma). For example, to measure circular DNA, cleaving can be performed to linearize the circular DNA so that they may be sequenced. Example cleaving techniques include restriction enzymes and transposases. Then, one or more criteria can be used to identify linearized DNA molecules, e.g., so as to differentiate from linear DNA molecules. An example criterion is mapping a pair of reversed end sequences to a reference genome. Another example criterion is identification of a cutting tag, e.g., associated with a restriction enzyme or an adapter sequence added by a transposase. Once circular DNA molecules (e.g., eccDNA and circular mitochondrial DNA) are identified, they may be analyzed (e.g., to determine a count, size profile, and/or methylation) to measure a property of the biological sample, including genetic properties and level of a disease.

Determining linear and circular forms of circulating nucleic acids

Growing community of inventors

Kowloon, China

Tsz Kwan Sin