Shiseido Company, Limited

Tsutomu Soma

Haruyo Yamanishi

Jiro Kishimoto

Shigeyoshi Fujiwara

Yuzo Yoshida

Yumiko Ishimatsu

Takayuki Arai

Shunsuke Iriyama

Masato Iino

Ritsuko Ehama

Kiichiro Yano

Ritsuro Ideta

The present disclosure relates to a serum-free medium for culturing of DS cells containing platelet derived growth factor (PDGF), or to a method for culturing of dermal sheath (DS) cells, using serum-free medium comprising PDGF.

Serum-free medium containing PDGF for DS cells

An agent comprising one or more components selected from among cowberry derivatives, mangosteen derivatives,root derivatives, inositol and inositol phosphate, as an active ingredient, is a novel agent that can effectively promote production of platelet-derived growth factor-BB (PDGF-BB), which contributes to production of mesenchymal stem cells and stabilization of stem cells.

Platelet-derived growth factor-BB production promotor, and mesenchymal stem cell production accelerator, stem cell stabilizer and dermal regenerator comprising the same

The present invention provides a method of producing a cell mass capable of serving as a primitive organ-like structure comprised of a plurality of somatic cell types of somatic origin, comprising: preparing cultures containing the plurality of types of somatic cells; mixing the plurality of types of somatic cell cultures followed by adding a Wnt signal activator to the mixed cell culture; subjecting the culture containing the Wnt signal activator to non-plate contact culturing over a predetermined time period; and replacing the medium of the culture cultured by the non-plate contact culturing with medium not containing Wnt signal activator and further culturing for a predetermined time period; wherein, at least one type of the plurality of somatic cells is maintained in an undifferentiated state.

Cell mass capable of serving as a primitive organ-like structure comprised of a plurality of cell types of somatic origin

Provided is a method for regenerating hair follicles by transplanting a composition containing CD36-expressing dermal sheath cells (DSc) to a human. The composition may be prepared by sorting CD36-expressing dermal sheath cells from a hair follicle tissue by use of a cell-sorting technique along with an antibody to CD36. The composition may also contain dermal papilla cells (DPc).

Method for regenerating hair follicles using CD36-expressing dermal sheath cells

This method, which screens drugs that activate the skin, is characterized by causing candidate drugs to act on vascular endothelial cells, and selecting drugs that enhance the expression of PDGF-BB by the cells as a skin activation agent.

Skin activation by acceleration of PDGF-BB activity

The invention provides a method of isolating dermal stem cells, having the steps of subjecting cells separated from the skin by enzyme treatment to suspension culture, and isolating cells positive for stem cell markers from the cultured cells.

Method of isolating dermal stem cells

Primitive organ-like structure comprising keratinocytes and hair papilla cells

An object of the present invention is to promote hair growth or hair regeneration by promoting formation and/or regeneration of hair follicles. A method is provided for promoting formation and/or regeneration of hair follicles, comprising maintaining or increasing expression of one or a plurality of genes involved in cell adhesion in dermal papilla cells.

Method for promoting hair growth or hair regeneration by maintaining or increasing expression of cell-adhesion factor

Disclosed is a cell transformed by a vector containing human Wnt3a gene, wherein the cell is selected from a group consisting of hair follicle-derived cells and prostate cancer-derived cells.

Cell transformed by human Wnt3a gene

The present invention provides a method for preparing a hair dermal papilla cell preparation comprising preparing a cell suspension by removing epidermal tissue from skin tissue and subjecting the resulting dermal tissue fraction to collagenase treatment, and cyropreserving the cell suspension to kill the follicular epidermal cells. The present invention also provides a composition for regenerating hair follicles comprising hair dermal papilla cell and epidermal cells, wherein the ratio of the number of hair dermal papilla cell to the number of epidermal cells is from 1:10 to 10:1.

Preparation method of a hair dermal papilla cell preparation, composition and method for regenerating hair follicles, and animal having regenerated hair follicles

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Yokohama, Japan

Tsutomu Soma