Kumiai Chemical Industry Co., Ltd.

National Institute of Agrobiological Sciences

Tohoku University

Tsutomu Shimizu

Koichiro Kaku

Kozo Nagayama

Kiyoshi Kawai

Atsunori Fukuda

Yoshiyuki Tanaka

Tsuyoshi Tanaka

Hiroyuki Yano

Seiichi Toki

Takashi Matsumoto

Kinya Toriyama

Yuji Misumi

Ayako Okuzaki

Katsushi Toyooka

Koji Yamaji

Jun Toyoshima

Ishizue Nakayama

Hidemitsu Nakamura

Hiroaki Ichikawa

Junko Horita

Kiyosumi Hori

Fumio Taguchi

Hiroaki Saika

To identify a cytochrome P450 involved in the detoxification and metabolism of a specific growth inhibitor. Provided is a gene encoding a cytochrome P450 classified into Indica rice-derived CYP72A31, comprising a polynucleotide encoding a protein comprising an amino acid sequence of SEQ ID NO: 2.

Gene encoding cytochrome P450, and use thereof

Disclosed is a gene coding for a protein (a) or (b) showing a high level of resistance to PC herbicides or sulfonylurea herbicides. The protein (a) consists of an amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8, and the protein (b) consists of an amino acid sequence derived from the amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8 by substitution, deletion or addition of at least one or more amino acids, has resistance to a pyrimidinyl carboxy herbicide, and has acetolactate synthase activity.

Gene coding for acetolactate synthase

Transformed cells are efficiently selected using a mutant ALS gene having high specificity to PC herbicides. The transformation method comprises the steps of: transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine; culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide; and wherein the gene coding for the mutant acetolactate synthase is used as a selection marker.

Method for transformation using mutant acetolactate synthase gene

The present invention provides a method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor, comprising the steps of: (a) and (b), which (a) identifies an amino acid in an amino acid sequence of a scytalone dehydratase isolated from the rice blast fungus, where the amino acid corresponds to valine at position 75 of SEQ ID NO: 4; and (b) assesses the sensitivity of the rice blast fungus to the scytalone dehydratase inhibitor, where if the amino acid identified in step (a) is methionine, the sensitivity of the rice blast fungus to The scytalone dehydratase inhibitor is assessed to be lower than that of a wild-type rice blast fungus which endogenously produces the scytalone dehydratase of SEQ ID NO: 4.

Method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor

The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion, substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.

Scytalone dehydrogenase gene showing tolerance to agricultural pesticide

The present invention provides methods for inducing tissue-specific expression, which includes preparing an expression vector having a promoter. The promoter includes any one of the following DNAs (a) to (c): (a) a DNA, which includes the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which includes the nucleotide sequence ranging from nucleotides 1344 to 2843 of SEQ ID NO: 1; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment including a nucleotide sequence fully complementary to the nucleotide sequence shown in SEQ ID NO: 1, wherein (a), (b) or (c) can control the transcription of a coding sequence located downstream thereof; and wherein the stringent conditions are 5&#xd7;SSC at 42&#xb0; C.; The expression vector, also, includes a coding sequence located downstream of the promoter. Additionally, the method includes introducing the expression vector into a plant cell to obtain a transgenic plant; wherein the promoter activity is lower in mature seeds or root bases than in plant tissues other than mature seeds or root bases. The present invention also provides transformants containing a promoter; the promoter includes any of the DNAs (a) to (c) described above.

Acetolactate synthase gene promoter

The present invention provides a gene encoding ALS protein which shows a extremely high level of resistance to pyrimidinyl carboxy herbicides. The gene of the present invention encodes an acetolactate synthase protein, for instance, according to SEQ ID NO:1, where the acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity.

Gene encoding acetolactate synthase

The present invention relates to a novel strain ofsp. D747 (deposited as FERM BP-8234) and methods for controlling plant diseases and insect pests, comprising administering cultures ofsp. D747 (including the viable bacteria) or viable bacteria isolated by culturing, on the plant parts such as roots, stems, leaves, seeds, and the like, or in the culture soil.

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The present invention provides a wettable composition for use in agriculture comprising microorganisms as an active ingredient, which can maintain a high survival rate of the same over long periods, and exhibit superior physical properties during applications, and provide a method for stably storing the wettable composition for use in agriculture for a long period of time. That is, the present invention corresponds to a wettable composition for use in agriculture comprising spores of filamentous fungi which are in the form of dried powders having a volume median diameter ranging from 2 &mgr;m to 10 &mgr;m, and having a ratio of (volume 90% diameter)/(volume 10% diameter;) of not more than 5.62, and an adsorbent having a water absorption ability in an amount of at least 1% by mass based on the total mass of the composition. The composition is prepared by pulverizing and mixing a mixture of spores of filamentous fungi, an adsorbent having a water absorption ability, and other additive components such as a surfactant, in an appropriate ratio of the same, by means of impact pulverization, and adjusting a moisture content so that the moisture content is less than 2.5% by mass based on the total mass of the composition. In addition, the composition is stored by packaging in a bag formed from a hermetic film.

Growing community of inventors

Shizuoka, Japan

Tsutomu Shimizu