International Reagents Corporation

Sysmex Corporation

Tsutomu Kakuyama

Koji Kishi

Koji Ochiai

Yasushi Shirahase

Yoshifumi Watazu

Yuzo Hasegawa

A method for quantitating a specific component in lipoproteins contained in a biological sample, for example, HDL (high-density lipoprotein), LDL (low-density lipoprotein) or VLDL (very low-density lipoprotein) by using a commonly employed automatic analyzer without centrifuging or making the reaction liquor cloudy due to complexes or aggregates. Namely, a controlling means, whereby an enzyme reaction can be carried out exclusively for the target component, is introduced into a method for enzymatically assaying a component in a specific lipoprotein fraction in the serum, thereby specifically assaying the component.

Reagent set and method for detecting cholesterol in a high-density lipoprotein or low density lipoprotein

There is provided a method for a selective assay of component, particularly cholesterol, in very low-density lipoprotein (VLDL) which is one of serum lipoproteins. In the assay, an enzymatic reaction of lipoprotein lipase (LPL) or cholesterol esterase (CE) which well reacts with high-density lipoprotein (HDL) and VLDL is carried out at least in the presence of calixarene or a salt thereof. It is also carried out in the presence of one or more substance(s) selected from albumin and basic amino acids in addition to calixarene or a salt thereof.

Method of analyzing components in biological samples

A method for assaying biological specimens for substances contained in the components of each specimen by the use of one or more kinds of calixarenes; and a reagent comprising one or more kinds of calixarenes. The method utilizes complexes formed by calixarenes and the components of biological specimens, and makes it possible to assay biological specimens for substances contained in the components of each specimen, for example, for cholesterol contained in high-density lipoprotein (HDL), without preliminary separation from the other components of the biological specimen. The method can be conducted by easy and simple operations and lessen measurement errors or problems caused by man, and permits continuous measurement with general-purpose automatic analyzing apparatuses and multi-channel measurement together with other test items.

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Kobe, Japan

Tsutomu Kakuyama