Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo

Hokkaido Sugar Co., Ltd.

Tsutomu Furuya

Tadao Tanimoto

Miyuki Kobayashi

Shinichi Ayabe

Masaru Yamada

Chikashi Izumi

Shigeyoshi Narita

Hiroshi Ishikawa

Echinatin glycosides are disclosed having the following formula ##STR1## wherein R.sub.5 and/or R.sub.6 is a glycosyl residue. These substances have pharmacological effects such as the inhibiting action of the echinatin-4'-glucoside on the growth of human lymphoblastoids. These compounds may be chemically synthesized by subjecting a compound of the following formula ##STR2## to reaction with a compound represented by the following formula ##STR3## in which R.sub.1 and/or R.sub.2 is a glycosyl residue or an acyl glycosyl residue, with the optional successive step of subjecting the resultant to reaction with a glycosyl halide or an acyl glycosyl halide.

Echinatin glycosides

A process for the biosynthesis of echinatin-4-glucoside comprises cultivating calli of Glycyrrhiza echinata L. (Leguminosae) in an appropriate nutrient medium. The echinatin-4-glucoside appears in both the calli and the culture broth and may be extracted and separated therefrom.

Echinatin glycosides and their preparation

A method for the recovery of refined .alpha.-galactosidase is disclosed. This method comprises the steps of bringing an .alpha.-galactosidase-containing liquid into contact with a weakly acidic cation-exchange resin in the presence of a buffer solution for thereby allowing .alpha.-galactosidase to be selectively adsorbed by said resin, desorbing the adsorbed .alpha.-galactosidase from said resin by use of a buffer solution of a type such that at least one of the two factors, concentration and pH, has a higher value than that of the buffer solution used in said adsorption and thereafter recovering the desorbed .alpha.-galactosidase.

Method for recovery of refined .alpha.-galactosidase

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Tokyo, Japan

Tsutomu Furuya