Albert Einstein College of Medicine

Other

Iowa State University Research Foundation Inc

Tsungda Hsu

William R Jacobs, Jr

Stoyan S Bardarov

Vasan Sambandamurthy

Svetoslav Bardarov, Legal Representative

Steven A Porcelli

F Chris Minion

John Chan

Kari Sweeney

Sheldon Morris

Dee Dao

Provided are mycobacteria deleted in at least a portion of a region 3 ESAT-6-like gene cluster. Also provided are mycobacteria comprising a mutation in an roc-1 gene. Additionally, vaccines comprising these mycobacteria are provided. Further provided are methods of making a recombinant, methods of inducing an immune response in a mammal, methods of inhibiting IL-12 production in a mammal, and methods of stimulating IL-12 production in a mammal. Vaccine adjuvants are also provided, as are methods of inducing immunity to a target antigen in a mammal.

Mycobacterial mutants inducing IL-12

Methods of treating a mammal that is deficient in CD4and/or CD8lymphocytes are provided. The methods comprise inoculating the mammal with an attenuated mycobacterium in thecomplex. In these methods, the mycobacterium comprises two deletions, wherein a virulent mycobacterium in thecomplex having either deletion exhibits attenuated virulence. Use of these mycobacteria for the manufacture of a medicament for the treatment of mammals deficient in CD4and/or CD8lymphocytes is also provided.

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Non-naturally occurring mycobacteria in thecomplex are provided. These mycobacteria have a deletion of an RD1 region or a region controlling production of a vitamin, and exhibit attenuated virulence in a mammal when compared to the mycobacteria without the deletion. Also provided are non-naturally occurring mycobacteria that have a deletion of a region controlling production of lysine, and mycobacteria comprising two attenuating deletions. Vaccines comprising these mycobacteria are also provided, as are methods of protecting mammals from virulent mycobacteria using the vaccines. Also provided are methods of preparing these vaccines which include the step of deleting an RD1 region or a region controlling production of a vitamin from a mycobacterium in thecomplex.

A Mycoplasma hyopneumoniae protein prepared by recombinant DNA or synthetic means, DNA sequences coding for the protein, an expression vector and transformed host containing the DNA sequences, a vaccine based on the protein, a vaccine based on the DNA sequences, methods of treating swine to prevent enzootic pneumonia using the vaccines, and diagnostic tests based on the protein or antibodies raised against it for detecting the presence of Mhyo infection in swine herds.

Recombinant mycoplasma hyopneumoniae vaccine

Growing community of inventors

Bronx, NY, United States of America

Tsungda Hsu