Mitsubishi Gas Chemical Company, Inc.

Nippon Suisan Kaisha, Ltd.

The Nisshin Oil Mills, Ltd.

Nippon Flour Mills Co., Ltd

Rinoru Oil Mills Co., Ltd.

Tsuneo Yamane

Kazuhiko Hata

Lekh Raj Juneja

Satoshi Sekiguchi

Kiyomi Furihata

Teizi Urakami

Shigeki Imagawa

Masaaki Kasai

Torakazu Tahara

Hideo Nakano

Hiroyuki Iesaka

Shunsaku Ueda

Yoshiharu Tokunaga

Shoichi Shimizu

Dongxiu Li

Masashi Ouchi

Reiko Okumura

Yugo Iwasaki

Yukiko Mizumoto

Takahiro Okada

Roxana Irimescu

The invention provides a process for producing 2-monoglycerides (2-MG) or triglycerides (TG), particularly triglycerides having polyunsaturated fatty acid (PUFA) residues in the sn-2 positions, at an extremely high purity and a high efficiency. The 2-MG is produced by conducting alcoholysis of a starting TG with a first 1,3-lipase. Then, the TG is produced by introducing fatty acid residues to the 1- and 3-positions of the produced 2-MG by using a second 1,3-lipase. The fatty acid residue in the 2-posiiton is preferably a residue of DHA, EPA or ARA. The 'first lipase' refers to one which is selective for the 1- and 3-positions and can act even on a long-chain fatty acid in alcoholysis, while the 'second lipase' refers to one which is selective for the 1- and 3-positions and can produce a TG through the reaction of a 2-MG with a fatty acid ester or a free fatty acid.

Process for the production of glycerides with lipases

Process for exchanging bases in phospholipids

A method for amplifying desired nucleic acid molecules by PCR which comprises the steps of isolating and purifying a group of nucleic acid molecules including desired nucleic acid molecules to be amplified, then carrying out PCR while establishing such a condition that the nucleic acid molecules capable of being amplified, except for primers, present in a PCR reaction solution are constituted by only the desired nucleic acid molecules and that the concentration of each primer used is limited to a level of not more than 100 nM; a method for producing a protein in a cell-free protein-synthesis system containing a cell-free extract which comprises using the nucleic acid of a single kind produced by the method described above and a method for establishing a protein library which comprises the steps of separately carrying out the method for amplifying desired nucleic acid molecules by PCR to obtain at least two kinds of nucleic acid molecules and separately carrying out the foregoing method for producing a protein using each of the at least two kinds of the amplified nucleic acids as a template to thus establish a protein library which comprises at least two kinds of proteins encoded by the resulting at least two nucleic acids respectively.

Method for amplifying nucleic acid molecules and method for synthesizing proteins

Disclosed is a process for the preparation of a copolymer, which comprises propagating cells of a bacterium having a capacity of producing poly-3-hydroxybutyrate mainly at a former stage, synthesizing and accumulating in the cells a copolymer comprising D-3-hydroxybutyrate and D-3-hydroxyvalerate by contacting the bacterium with a mixture of a carbon source utilizable by the bacterium and a primary alcohol having 3 to 7 carbon atoms, or with a primary alcohol having 3 to 7 carbon atoms, at a latter stage, and recovering the copolymer from the cells. According to this process, a copolymer comprising D-3-hydroxybutyrate and D-3-hydroxyvalerate can be manufactured in a large quantity at a low cost.

Process for preparation of copolymer

A method for preparing highly purified phosphatidylinositol comprises the steps of treating a mixed phospholipid with a phospholipase D, then treating the product with an alkali or acidic phosphatase and separating unreated phosphatidylinositol from the reaction mixture. The method makes it possible to isolate phosphatidylinositol useful in various fields in a high purity in the order of 80 to 99%.

Method for preparing highly purified phosphatidylinositol

Growing community of inventors

Nagoya, Japan

Tsuneo Yamane