Dna Vec Research Inc.

Shionogi Company Limited

Tsuneaki Sakata

Mamoru Hasegawa

Atsushi Kato

Yoshiyuki Nagai

Tatsuo Shioda

Fukashi Murai

Kohji Hanasaki

Ryuji Suzuki

Saichi Matsumoto

Hirokuni Jyoyama

Makoto Asakawa

Kenzo Koizumi

Shinji Kakudo

Hiroyuki Mano

Toshiaki Tabata

Tatsuo Muramatsu

A method for regenerating Sendai virus particles by transfecting the Sendai virus genome to a host expressing all genes for the initial viral replication has been developed, enabling the genetic manipulation of Sendai virus and effective utilization of said virus as the vector.

Recombinant sendai virus

A method for generating Sendai virus particles by transfecting the Sendai virus genome to a host expressing all genes for the initial viral replication has been developed, enabling the genetic manipulation of Sendai virus and effective utilization of said virus as the vector.

Recombinant Sendai virus

Antirheumatic agent

A method for reconstituting Sendai viral particles by transfecting Sendai virus to a host expressing all genes for the initial replication has been developed, enabling the Production of negative strand RNA vectors highly useful for practical applications.

Negative strand RNA viral vector having autonomous replication capability

A method for reconstituting Sendai viral particles by transfecting Sendai virus to a host expressing all genes for the initial replication has been developed, enabling the production of negative strand RNA vectors highly useful for practical applications.

A gene is transferred to the early stage chicken embryo by electroporation with placing the electrode tips to hold both ends of the embryo. Transfer of the desired gene to the early stage embryo was detected using the &bgr;-galactosidase gene of as a reporter gene. The expression of the gene in the early stage embryo of chicken was thus confirmed. This method for transferring a gene into undifferentiated cells is simple to implement, highly efficient in transferring a gene, and applicable to various animal species.

In vivo electroporation method for early stage embryo of chickens

Antirheumatic

The present invention provides a DNA having promoter activity of Tec tyrosine kinase and a vector having incorporated within it the promoter to thereby enable a high level expression of an exogenous gene in hematopoietic stem cells and hepatic cells.

Promoter

TEC promoter

Growing community of inventors

Osaka, Japan

Tsuneaki Sakata