Eiken Kagaku Kabushiki Kaisha

Toyo Seikan Kaisha, Ltd.

National Institute of Infectious Diseases

Tsugunori Notomi

Toshihiro Yonekawa

Hidetoshi Kanda

Tetsu Hase

Kentaro Nagamine

Yasuyoshi Mori

Shingo Saito

Tomoyuki Shibata

Harumi Minekawa

Yasuyuki Takeuchi

Yoshiaki Seto

Yutaka Kubota

Tsuyoshi Shindome

Norimitsu Hosaka

Norihiro Tomita

Teijirou Kanada

Natsuki Masuya

Takato Odagiri

Yoko Kuzuhara

Youichi Takano

Akira Onishi

The present invention relates to an oligonucleotide having a novel structure and a method of synthesizing nucleic acid by using the same as a primer. This oligonucleotide is provided at the 5'-side of the primer with a nucleotide sequence substantially the same as a region synthesized with this primer as the origin of synthesis. The present invention realizes synthesis of nucleic acid based on an isothermal reaction with a simple constitution of reagents. Further, the present invention provides a method of synthesizing highly specific nucleic acid on the basis of this method of synthesizing nucleic acid.

Method of synthesizing nucleic acid

The object is to remove substances that can inhibit nucleic acid amplification from biological samples and nucleic acid extracts, and to allow convenient and accurate evaluation of the presence or absence of a target nucleic acid or the expression level of a target gene in a biological sample, by nucleic acid amplification means employing an enzyme. The invention provides a method for preparation of a sample for use in nucleic acid amplification, to be used for amplification of nucleic acid in a biological sample, which method comprises an extraction step in which a nucleic acid extraction reagent is added to the biological sample to obtain a nucleic acid extract, and a purification step in which the nucleic acid extract is contacted with zeolite to obtain a nucleic acid amplification sample suitable for nucleic acid amplification, to allow nucleic acid detection at high sensitivity.

Method and kit for preparation of sample for use in nucleic acid amplification

A compound container has a container main body forming a first accommodation chamber, an auxiliary container forming a second accommodation chamber, a mounting part on which the auxiliary container is mounted in the container main body, a cutting part which cuts a second accommodation chamber partition wall which partitions a part of the second accommodation chamber formed in the auxiliary container, a contents outlet port in the container main body, and an opening pouring element mounted on the contents outlet port and having another cutting part which cuts a first accommodation chamber partition wall which partitions a part of the first accommodation chamber formed in the container main body.

Compound container and pouring-out method

The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.

Method of nucleic acid amplification and measuring reagent and reagent kit therefor

The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis.

Method of detecting H5 or H7 avian influenza virus

The present invention provides a nucleic acid synthesis method which involves the step of incubating a double-stranded nucleic acid template under conditions that ensure a complementary strand synthesis reaction using a primer as an origin. This method involves the step of placing a region, to which a primer capable of isothermally amplifying the template nucleic acid will anneal, in a condition that ensures base pairing, using an arbitrary primer. The arbitrary primer initiates the complementary strand synthesis reaction, using the double-stranded nucleic acid as a template and DNA polymerases catalyzing the complementary strand synthesis reaction which comprises the destabilization of the double-stranded nucleic acid and strand displacement, thereby providing a region that can undergo base pairing.

Method of amplifying nucleic acid by using double-stranded nucleic acid as template

The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3'-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.

Method for detecting mutations and/or polymorphisms

Method of detecting variation or polymorphism

The present invention relates to an oligonucleotide having a novel structure and a method of synthesizing nucleic acid by using the same as a primer. This oligonucleotide is provided at the 5&prime;-side of the primer with a nucleotide sequence substantially the same as a region synthesized with this primer as the origin of synthesis. The present invention realizes synthesis of nucleic acid based on an isothermal reaction with a simple constitution of reagents. Further, the present invention provides a method of synthesizing highly specific nucleic acid on the basis of this method of synthesizing nucleic acid.

Process for synthesizing nucleic acid

Growing community of inventors

Tochigi, Japan

Tsugunori Notomi