Beckman Coulter, Inc.

Coulter International Corp.

Tsong-Tseh Tsay

Michael L Bell

Stephen L Pentoney, Jr

Yuan Lin

Josephine M Michael

Yagang Liu

Charles L Dodson

Chiranjit Deka

Robert J Obremski

Gene G-y Shen

Tung Rung Wang

John Warwick Silzel

Stephen Szydlo

Song Y Lee

Bibijana Cercek

Zhou Shaomin

An analyte detection system utilizing a combination of fluorescent labels for labeling particles and an analyte specific fluorescent analyte detection dye. The particles contain a combination of fluorescent labels for coding the particles and an analyte specific fluorescent dye. The particles can be used to identify and quantify analytes in an analytical sample by reaction of the analytical sample with the particles. An analytical device can identify the particles according to the combination of fluorescent labels. The device can then correlate the identified particle with the analyte specific fluorescent analyte detection dye. Multiple subpopulations of particles can be used to identify and quantify multi-analytes in a single analytical sample. Near infrared (NIR) fluorescent labels useful in the detection system are also provided.

Analyte detection system

A microscale binding assay, analyte binding array, and kits are disclosed, which exploit the mass action law to harvest analyte from a liquid sample. This is achieved by fabrication of sorbent zones having up to ten times the binding capacity per unit area generally obtained on polystyrene microtiter plates. The resulting arrays substantially deplete the liquid solution of analyte during incubation. Accordingly, the assays respond to total mass of analyte in the sample, not analyte concentration. This approach, coupled with direct fluorescence detection in the NIR, yields maximal signal intensity and low background for optimal sensitivity.

Detection of very low quantities of analyte bound to a solid phase

Dyes and methods of reticulocyte enumeration

Growing community of inventors

Hsinchu, Taiwan

Tsong-Tseh Tsay