Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Danstar Ferment Ag

Enchi Corporation

Dartmouth College

Universiteit Stellenbosch

Lallenmend Hungary Liquidity Management LLC

Trisha Barrett

Aaron Argyros

Nicky Caiazza

Charles F Rice

Ryan Skinner

Arthur J Shaw, Iv

Anne K Warner

Frank Agbogbo

John E McBride

Allan C Froehlich

Erin Wiswall

Mark Mellon

Riaan Den Haan

Emily Stonehouse

Haowen Xu

Chhayal V Gandhi

Shital A Tripathi

Kristen M Deleault

John S Bardsley

Kevin S Wenger

Indraneel Shikhare

Vineet B Rajgarhia

Abigail Foster

Jennifer Gosselin

Yin-Ying Chiu

Alan Belcher

Elena E Brevnova

Heidi H Hau

William Ryan Sillers

D Aaron Argyros

Johannes Pieter Van Dijken

Michelle Oeser

Janet Fisher

Daniel G Olson

Christopher D Herring

Yu Deng

Stephen R Rogers

Dave Hogsett

Elena Brevnova

Heidi Lau

Arthur J Shaw

John E Mcbride

Abigail S Foster

Arthur J Shaw Iv

Iv Shaw Arthur J

The present disclosure concerns the use of specific genetic modification(s) for improving sulfite tolerance in recombinant yeast host cells. The genetic modification(s) is (are) designed to allow the expression of an heterologous transcription factor favoring the expression of a SSU1 polypeptide and/or the expression of an heterologous SSU1 polypeptide in the recombinant yeast host cell(s).

Sulfite tolerance in recombinant yeast host cells

The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).

Limiting yeast-produced trehalose in fermentation

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention provides for a mechanism to reduce glycerol production and increase nitrogen utilization and ethanol production of recombinant microorganisms. One aspect of this invention relates to strains ofwith reduced glycerol productivity that get a kinetic benefit from higher nitrogen concentration without sacrificing ethanol yield. A second aspect of the invention relates to metabolic modifications resulting in altered transport and/or intracellular metabolism of nitrogen sources present in corn mash.

Methods for regulating nitrogen metabolism during the production of ethanol from corn by metabolically engineered yeast strains

The present invention provides for a mechanism to completely replace the electron accepting function of glycerol formation with an alternative pathway to ethanol formation, thereby reducing glycerol production and increasing ethanol production. In some embodiments, the invention provides for a recombinant microorganism comprising a down-regulation in one or more native enzymes in the glycerol-production pathway. In some embodiments, the invention provides for a recombinant microorganism comprising an up-regulation in one or more enzymes in the ethanol-production pathway.

Electron consuming ethanol production pathway to displace glycerol formation in

The present disclosure relates to recombinant yeast strains capable of maintaining their robustness at high temperature as well as recombinant proteins expressed therefrom. The present disclosure also provides methods for using the recombinant yeast strain for making a fermentation product. The present disclosure further process a process for making recombinant yeast strains capable of maintaining their robustness at high temperature.

Yeast strains for the expression and secretion of heterologous proteins at high temperatures

The present invention provides for novel metabolic pathways to reduce or eliminate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising a deletion of one or more native enzymes that function to produce glycerol and/or regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more heterologous enzymes that function to regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and/or heterologous enzymes is activated, upregulated or downregulated.

Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors

The present invention provides for a mechanism to reduce glycerol production and increase nitrogen utilization and ethanol production of recombinant microorganisms. One aspect of this invention relates to strains ofwith reduced glycerol productivity that get a kinetic benefit from higher nitrogen concentration without sacrificing ethanol yield. A second aspect of the invention relates to metabolic modifications resulting in altered transport and/or intracellular metabolism of nitrogen sources present in com mash.

Growing community of inventors

Bradford, VT, United States of America

Trisha Barrett