Codexis, Inc.

Trish Choudhary

Fernando Valle

Jonathan Vroom

Lorraine Joan Giver

Louis Clark

Richard John Fox

Kristian Karlshoej

Douglas A Hattendorf

Kaman Chan

Yoram Barak

Svetlana Balatskaya

Daniel Held

Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a &#x3b2;-ketoacyl acyl carrier protein synthase.

Production of fatty alcohols from engineered microorganisms

Region shuffling methods for efficiently introducing diversity and exploring sequence space are described. Libraries produced directly from these methods contain high fractions of protein variants harboring multiple beneficial mutations. Typically, the methods produce these variants efficiently without the need for sequencing beneficial mutants identified at intermediate stages of the process.

Protein variant generation by region shuffling

Growing community of inventors

Foster City, CA, United States of America

Trish Choudhary