University of California

National Technology & Engineering Solutions of Sandia, LLC

Other

Trent Russell Northen

Benjamin P Bowen

Matthew Paul Greving

Markus De Raad

Kai Deng

Jian Gao

Anup Kumar Singh

Todd A Duncombe

Lloyd T Cornmesser

Peter F Andeer

Katherine B Louie

Marc K Hellerstein

Seema Singh

N Louise Glass

Noel Seunghyun Ha

Oliver Ruebel

Xiaoliang Cheng

Curt R Fischer

Wolfgang E Reindl

Marion Leclerc

Jens Heller

Kyle M Lewald

Lauren Jabusch

Peter Kim

Onur Erbilgin

Joshua Vance Heinemann

The present invention provides for a composition for ionizing a target comprising: a substrate comprising a plurality of nanoparticles bound to a surface of the substrate, which allows one to directly extract undesired contaminants during passive sample drying without require manual interventions, such as washing and vortexing.

Self-assembled nanoparticle film for nanostructure-initiator mass spectrometry (NIMS)

This disclosure provides systems, methods, and apparatus related to the determination of ecological processes. In one aspect, a device includes a base and a substrate in contact with a first side of the base. The base defines a stem port. The substrate and the first side of the base define a chamber. The chamber includes a root chamber and a first nutrient chamber. The root chamber and the first nutrient chamber are separated by a first mesh having openings of about 1 micron to 300 microns. The device is operable to house a plant, with roots of the plant being in the root chamber, and a stem of the plant passing through the stem port.

Devices for studying plant-fungus interactions and for imaging plant roots

Disclosed herein are systems and methods for associating metabolites with genes. In some embodiments, after potential metabolites are identified based on spectroscopy data of the content of an organism, possible reactions capable of producing the potential metabolites are determined. The possible reactions are compared to gene sequences in a database, and an association score for the likelihood that a gene sequence is related to the potential metabolites is calculated.

Metabolite, annotation, and gene integration system and method

Disclosed herein are methods for tracking solutions, (e.g., reaction conditions in solutions). In some embodiments, the method comprises: contacting a first lanthanide-chelator complex to a first solution to generate a first barcoded solution, wherein the first lanthanide-chelator complex comprises a first lanthanide chelated by a first chelator; contacting a second lanthanide-chelator complex to a second solution to generate a second barcoded solution, wherein the second lanthanide-chelator complex comprises a second lanthanide chelated by a second chelator; mixing the first barcoded solution and the second barcoded solution to form one or more mixtures; and identifying the first lanthanide ions in the mass spectrum and the second lanthanide ions in the mass spectrum to track the condition of each of the one or more mixtures.

Lanthanide-chelator combinatorial barcoding

This disclosure provides systems, methods, and devices related to the study of ecological processes. In one aspect, a device includes a base, a substrate, and an enclosure. The substrate is in contact with a first surface of the base. The substrate and the base define a root chamber. The enclosure is in contact with a second surface of the base. The base and the enclosure define a growth chamber. The base defines a stem port connecting the root chamber and the growth chamber. The base further defines a first port in fluid communication with the root chamber and a second port in fluid communication with the root chamber. The device is operable to contain a plant, roots of the plant being in the root chamber, a stem of the plant passing through the stem port, and leaves of the plant being in the growth chamber.

Ecosystem device for determining plant-microbe interactions

Disclosed herein include systems, devices, and methods for droplet deposition for mass spectrometry. In some embodiments, a microfluidic device comprising wells is reversibly sealed to a mass spectrometry surface and used to deposit contents of droplets (e.g., enzymes and substrates), or products thereof, onto the mass spectrometry surface. The contents of droplets can be analyzed by laser desorption/ionization to, for example, identify a substrate of an enzyme or an enzyme capable of catalyzing a substrate to a product.

Picoliter droplet sample processing and deposition for mass spectrometry

Disclosed herein are methods for identifying one or more diseased cells in a subject. Some embodiments include providing a biological sample derived from a subject, analyzing the biological sample by mass spectrometry, and determining the abundance of one or more lipids in the biological sample, wherein an altered abundance of the one or more lipids in the biological sample, as compared to a reference level, indicates a presence of one or more diseased cells in the subject from which the biological sample is derived.

Use of lipid particles in medical diagnostics

Disclosed herein are methods, systems and compositions for determining substrate specificity of an enzyme. The disclosed methods, systems and compositions can be used for identifying enzymes capable of modifying substrates of interest and/or quantifying enzymatic activity.

Methods for determining gene functions

Disclosed herein are compositions for ionizing a target and methods for making the compositions. In some embodiments, the compositions can include a structured substrate having a plurality of upright surface features, for example, microscale or nanoscale pillars, in contact with an initiator. Also disclosed herein are methods for ionizing targets.

Fabrication of mass spectrometry surface

A method for rapid, high throughput screening of the activities of enzymes, especially ligninases and its enzyme cocktails, using nanostructure initiator mass spectrometry (NIMS) surfaces, substrates and methodology.

Determination of ligninases activities by nano-structure initiator mass spectrometry

Disclosed herein are systems, methods, and devices for determining plant-microbe interactions. In some embodiments, the device comprises: a root chamber configured to allow a root of a plant to grow, wherein the root chamber is connected with: an inlet channel for introducing a medium into the root chamber, an outlet channel for collecting plant exudates and metabolites, and a plant reservoir for the plant shoot of the plant to grow.

Ecosystem for determining plant-microbe interactions

Disclosed herein are devices and methods for detecting compounds in droplets using mass spectrometry. In some embodiments, the device comprises: a microfluidics-MS (microMS) device, wherein the microMS device comprises: a droplet-to-digital microfluidic device, wherein the droplet-to-digital microfluidic device comprises: a glass layer; an electrode layer comprising chrome electrodes etched onto one side of the glass layer; a dielectric layer configured for electrowetting; and a microfluidics layer comprising channels, pockets, and a droplet generator, for example a T-junction droplet generator, wherein the pockets are connected to the channels; and a mass spectrometry plate, wherein the mass spectrometry plate is reversibly sealed to the microfluidic device.

Detecting compounds in microfluidic droplets using mass spectrometry

The disclosure herein includes compositions and methods for ionizing targets and methods for making the compositions. In some embodiments, the compositions can include a porous substrate that has been etched for a desired average pore size, a desired porosity, or both for detection of one or more targets of interest. Also disclosed herein are methods for using the composition to ionize targets.

Detection of analytes using porous mass spectrometry surface

Provided herein are methods for measuring molecular flux rates of molecules of interest in a tissue sample in spatially-organized manner and generating output (e.g., an image, a heat map, a contour map, a table or a database) representing the molecular flux rates of each spatially-defined location of the sample. Provided herein are also the output, as well as systems and computer-readable medium with computer-executable instructions for determining molecular flux rates of molecules of interest in the sample.

Metabolic flux measurement, imaging and microscopy

Disclosed herein are methods, compositions and systems for analyzing and detecting enzyme activity. For examples, methods, compositions and systems for parallel detection and analysis of enzymatic activities of enzymes in complex biological mixtures are provided.

Growing community of inventors

Walnut Creek, CA, United States of America

Trent Russell Northen