Promega Corporation

University Technololy Corporation

Trent Gu

James Robert Hartnett

John William Shultz

Martin Ken Lewis

Donna M Leippe

Michelle N Mandrekar

Roy Welch

Christine Ann Andrews

Daniel Kephart

Richard Byron Rhodes

Keith V Wood

Fen Huang

Ryan J Olson

Min Han

Satoshi Orita

The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases.andpossess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.

Method of reverse transcription

The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms ofDNA polymerase in sequencing, reverse transcription, and amplification reactions.

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Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.

Detection of nucleic acid hybrids

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of predetermined nucleic acid target sequences using a multiplex assay format. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, genotyping, medical marker diagnostics, and the like.

Multiplex method for nucleic acid detection

The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species . The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of DNA polymerase in sequencing, reverse transcription, and amplification reactions.

Thermophilic DNA polymerases from Thermoactinomyces vulgaris

The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. and possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined endogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.

Nucleic acid detection

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined exogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, determination of viral load, genotyping, medical marker diagnostics, and the like.

Exogenous nucleic acid detection

The present invention relates to Ras suppressors, in particular the Ras suppressor SUR-5. The present invention provides isolated nucleotide sequence encoding SUR-5, isolated SUR-5 peptides, antibodies that specifically bind SUR-5, methods for the detection of SUR-5, methods for producing SUR-5 transgenic animals, non-human animals expressing SUR-5, and methods for screening compounds for the ability to alter SUR-5 associated signal transduction.

Growing community of inventors

Madison, WI, United States of America

Trent Gu