University of Maine System

The Jackson Laboratory

University of Utah Research Foundation

Isis Innovation Limited

Oxford University Innovation Ltd

Travis J Gould

Joerg Bewersdorf

Sam Hess

Manuel F Juette

Erik Jorgensen

Martin James Booth

Martin Booth

Brian Thomas Bennett

Daniel Burke

Mudalige Siyath Gunewardene

Sam T Hess

Aberrations in stimulated emission depletion microscopy are corrected using an adaptive optics approach using a metric which combines both image sharpness and brightness. Light modulators () are used to perform aberration correction in one or more of the depletion path (), the excitation path (), or the emission path from sample to detector.

Stimulated emission depletion microscopy

A system () and method for creating three dimensional images using probe molecules is disclosed and described. A sample is mounted on a stage (). The sample has a plurality of probe molecules. The sample is illuminated with light, causing the probe molecules to luminesce. The probe luminescence can be split into at least four paths corresponding to at least four detection planes corresponding to object planes in the sample. The at least four detection planes are detected via a camera (). Object planes in corresponding recorded regions of interest are recorded in the camera (). A signal from the regions of interest is combined into a three dimensional image.

Microscopy system and method for creating three dimensional images using probe molecules

A microscopy system is configured for creating 3D images from individually localized probe molecules. The microscopy system includes a sample stage, an activation light source, a readout light source, a beam splitting device, at least one camera, and a controller. The activation light source activates probes of at least one probe subset of photo-sensitive luminescent probes, and the readout light source causes luminescence light from the activated probes. The beam splitting device splits the luminescence light into at least two paths to create at least two detection planes that correspond to the same or different number of object planes of the sample. The camera detects simultaneously the at least two detection planes, the number of object planes being represented in the camera by the same number of recorded regions of interest. The controller is programmable to combine a signal from the regions of interest into a 3D data.

3D biplane microscopy

Growing community of inventors

New Haven, CT, United States of America

Travis J Gould