Mycogen Corporation

Hycogen Corporation

Tracy Ellis Michaels

Kenneth Edwin Narva

Jewel M Payne

H Ernest Schnepf

George E Schwab

Guy A Cardineau

Mark E Knuth

Michael R Pollard

Kendrick Akira Uyeda

Christine Julie Stalder

Stacey Finstad Lee

Joanna Dojillo

Gregory A Bradfisch

Rod Aaron Herman

Lisa M Stamp

Paula Burmeister

Paula Diehl

Judy Muller-Cohn

Luis Foncerrada

Jenny M Fu

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal proteins have molecular weights of approximately 40-50 kDa and of approximately 10-15 kDa.

Pesticidal proteins

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal protein have molecular weights of approximately 40&#x2013;50 kDa and of approximately 10&#x2013;15 kDa.

The subject invention concerns new classes of insecticidal proteins obtainable from, and polynucleotides that encode these proteins. The subject invention also includes transgenic cells and plants that produce these proteins. The proteins are preferably in the 10-15 kDa and 40-50 kDa size range.

Pesticidal toxins

Disclosed claimed are toxins produced by novel isolates designated . PS92J, . PS196S1, . PS201L1, and . PS201T6, which have dipteran and/or corn rootworm activity. Thus, the isolates, or mutants therof, can be used to control such pests. Further, claimed are novel genes encoding these &dgr;-endotoxins, which can be expressed in other hosts. Expression of the &dgr;-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.

Bacillus thuringiensis isolates and toxins

The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encoded these toxins. Also described are novel pesticidal isolates of

The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encode these toxins. Also described are novel pesticidal isolates of

Disclosed and claimed are approximately 130 kDa toxins produced by Bacillus thuringiensis PS201T6, which can be used to control pests. Further, claimed are novel genes encoding these .delta.-endotoxins, which can be expressed in other hosts. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts results in the control of susceptible insect pests in the environment of such hosts.

Polynucleotides encoding a 130 kDa pesticidal protein from Bacillus

The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encode these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.

The subject invention concerns Bacillus thuringiensis microbes with activity against select coleopteran pests, e.g., Diabrotica sp., Hypera sp., and various flea beetles. For example, the B.t. isolates of the invention are active against alfalfa weevils (AW, Hypera brunneipennis), rape flea beetles (RFB, Phyllotreta cruciferae), and corn rootworms (CRW, Diabrotica undecimpunctata undecimpunctata). Thus, these microbes can be used to control these pests. Further, genes encoding toxins active against these pests can be isolated from the B.t. isolates and used to transform other microbes. The transformed microbes then can be used to control susceptible coleopteran pests. In preferred embodiments, a gene from PS140E2 is used to transform plants so that the transformed plants are resistant to flea beetles of the genus Phyllotreta.

Bacillus thuringiensis isolates, toxins, and genes selectively active

Disclosed and claimed are toxins produced by novel Bacillus thuringiensis insolates designated B.t. PS92J, B.t. PS196S1, B.t. PS201L1; and B.t. PS201T6, which have dipteran and/or corn rootworm activity. Thus, the insolates, or mutants thereof, can be used to control such pests. Further, claimed are novel genes encoding these .delta.-emdotoxins, which can be expressed in other hosts, Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.

The subject invention concerns Bacillus thuringiensis microbes with activity against select coleopteran pests e.g., Diabrotica sp., Hypera sp., and various flea beetles. For example, the B.t. isolates of the invention are active against alfalfa weevils (AW, Hypera brunneipennis), rape flea beetles (RFB, Phyllotreta cruciferae), and corn rootworms (CRW, Diabrotica undecimpunctata undecimpunctata). Thus, these microbes can be used to control these pests. Further, genes encoding toxins active against these pests can be isolated from the B.t. isolates and used to transform other microbes. The transformed microbes then can be used to control susceptible coleopteran pests.

Growing community of inventors

Escondido, CA, United States of America

Tracy Ellis Michaels