Pioneer Hi-bred International, Inc.

Tracy A Rood

Jon Duvick

Jonathan P Duvick

Xun Wang

Joyce R Maddox

Benjamin A Bowen

Jacob T Gilliam

Aragula Gururaj Rao

Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

Fumonisin detoxification compositions and methods

The present invention provides a bacterial microorganism having the ability to degrade or detoxify beauvericin or derivatives or analogs of beauvericin. The present invention further provides a method for detoxification of plants pre- or post-harvest using microbes having the ability to degrade or detoxify beauvericin or derivatives or analogs of beauvericin.

Beauvericin detoxification method using bacteria

Beauvericin detoxification compositions and methods

The present invention provides a bacterial microorganism, Ochrobactrum anthropi, having the ability to degrade or detoxify moniliformin or structurally related mycotoxins. The present invention further provides a method for detoxification of grain pre- or post-harvest using Ochrobactrum anthropi having the ability to degrade or detoxify moniliformin or derivatives or analogs of moniliformin.

Ochrobactrum anthropi bacteria which degrade moniliformin

The present invention provides a bacterial microorganism having the ability to degrade or detoxify zearalenone or derivatives or analogs of zearalenone. The present invention also provides a method for the isolation and utilization of a zearalenone-degradation gene encoding a gene product having the ability to degrade or detoxify zearalenone or derivatives or analogs of zearalenone. In another embodiment, the present invention provides for the generation of transformants into which the zearalenone-degradation gene has been introduced, thereby providing the ability to degrade or detoxify zearalenone or derivatives or analogs of zearalenone to said transformants. The present invention further provides a method for detoxification of plants pre- or post-harvest using microbes having the ability to degrade or detoxify zearalenone or derivatives or analogs of zearalenone. The invention also provides a method for detoxification of plants pre- or post-harvest using the zearalenone-degradation gene.

Zearalenone detoxification compositions and methods

The present invention provides a bacterial microorganism Ochrobactrum having the ability, to degrade or detoxify moniliformin or structurally related mycotoxins. The present invention further provides a method for detoxification of grain pre- or post-harvest using Ochrobactrum bacterium having the ability to degrade or detoxify moniliformin or derivatives or analogs of moniliformin and wherein the preferred bacterium is Ochrobactrum anthropi.

Method of degrading moniliformin with ochrobactrum

The present invention provides a bacterial microorganism of the Rhodococcus or Nocardia species having the ability to degrade zearalenone.

CMIII, a small, basic maize seed peptide has been found to have antimicrobial properties. In a preferred embodiment, plant resistance to diseases caused by plant pathogens which are susceptible to CMIII is produced by inserting into the cells of a plant a gene whose expression causes production CMIII in antimicrobial amounts.

Antimicrobial peptides and plant disease resistance based thereon

Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on flumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzyme and the gene responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for furaonisin-resistance can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain processing and in animal feed and rumen microbes are also disclosed.

Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin. Using this method, several organisms have been identified. These organisms can be used to isolate the enzyme and the gene responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin-resistance can be used to transform plant cells normally susceptible to Fusarium infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin.

Growing community of inventors

Johnston, IA, United States of America

Tracy A Rood