Sanraku-ocean Co., Ltd.

Other

Toyokazu Nishino

Sawao Murao

Toshikazu Oki

Yasutaro Hamagishi

A method of inactivating cytosolic aspartate aminotransferase isozyme comprises addition of a specific inhibitory enzyme. A method for the fractional determination of aspartate aminotransferase isozyme activities, which comprises (a) inactivating the cytosolic aspartate aminotransferase isozyme in a reaction mixture containing the specimen by the addition of a specific inhibitory enzyme, followed by determination of the residual mitochondrial aspartate aminotransferase isozyme activity, and (b) determination of the cytosolic isozyme activity by subtracting the activity of mitochondrial isozyme determined in (a) from the total activity of aspartate aminotransferase isozymes. A cytosolic aspartate aminotransferase isozyme inhibiting composition contains an effective cytosolic aspartate aminotransferase isozyme inhibitory amount of a specific inhibitory enzyme.

Method for fractional determination of aspartate aminotransferase

A process for preparing an enzyme capable of inactivating cytosolic aspartate aminotransferase isozyme. The process comprises cultivating a strain of Streptomyces violaceochromogenes species capable of producing said enzyme to produce and accumulate said enzyme and recovering said enzyme from the culture.

Process for producing enzyme capable of inactivating cytosolic aspartate

New purine nucleoside 5'-phosphate (mono, di or tri) 3'(2' )-diphosphates and the sodium, lithium and potassium salts thereof are elaborated by the enzymatic transfer of pyrophosphoryl group from ATP (adenosine triphosphate), dATP (deoxyadenosine triphosphate) and pppApp (adenosine 5'-triphosphate 3'(2') diphosphate) to specified 5'-purine nucleotides, and a new nucleotide pyrophosphotransferase used for this enzyme reaction is produced by actinomycetes and other microorganisms and recovered from the culture filtrate and mycelium by the conventional methods for recovering enzymes.

Nucleotide pyrophosphotransferase and method of preparation

New purine nucleoside 5'-phosphate (mono, di or tri) 3'(2')-diphosphates and the sodium, lithium and potassium salts thereof are elaborated by the enzymatic transfer of pyrophosphoryl group from ATP (adenosine triphosphate), dATP (deoxyadenosine triphosphate) and pppApp (adenosine 5'-triphosphate 3'(2') diphosphate) to specified 5'-purine nucleotides, and a new nucloetide pyrophosphotransferase used for this enzyme reaction is produced by actinomycetes and other microorganisms and recovered from the culture filtrate and mycelium by the conventional methods for recovering enzymes. These compounds are useful for treating leukemia L1210 in mice.

Purine nucleoside 5'-phosphate (mono, di or tri) 3'(2')-diphosphates and

New purine nucleoside 5'-phosphate (mono, di or tri) 3'(2')-diphosphates and the sodium, lithium and potassium salts thereof are elaborated by the enzymatic transfer of pyrophosphoryl group from ATP (adenosine triphosphate), dATP (deoxyadenosine triphosphate) and pppApp (adenosine 5'-triphosphate 3'(2')diphosphate) to specified 5'-purine nucleotides, and a new nucleotide pyrophosphotransferase used for this enzyme reaction is produced by actinomycetes and other microorganisms and recovered from the culture filtrate and mycelium by the conventional methods for recovering enzymes.

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Hikisokita, Japan

Toyokazu Nishino