Osaka University

Japan As Represented by the Director-general of National Institute of Infections Diseases

Toyokazu Ishikawa

Masahide Ishibashi

Toshiyuki Onishi

Isao Fuke

Michiaki Takahashi

Hideo Goda

Takeshi Nabeshima

Shinichi Miyake

Kouichi Morita

Hidehiro Takahashi

Tadashi Imagawa

Morita Kouichi

Asato Kojima

Hironori Yoshii

The present invention provides virus-like particles (VLP) highly secreting or producing signal peptide obtained by altering a signal sequence derived from West Nile virus (WNV), the signal peptide, a WNV VLP secretion expression vector containing a nucleic acid encoding prM protein and E protein, a WNP VLP highly secreting or producing animal cell line harboring the vector, a WNV vaccine containing WNV VLP obtained by the cell line as an active ingredient, and a WNV DNA vaccine containing the VLP secretion expression vector as an active ingredient.

West Nile virus prM-E signal peptide facilitating the efficient assembly, maturation, and release of virus-like particles (VLPs)

A nucleic acid molecule containing nucleotide sequences that encode the capsid protein, pre-membrane protein and non-structural protein of Japanese encephalitis virus, and a nucleotide sequence that encodes the envelop protein of a second flavivirus, wherein the nucleotide sequence(s) that encode(s) the pre-membrane protein and/or non-structural protein of Japanese encephalitis virus contain(s) nucleotide mutations that produce one or more amino acid mutations that attenuate the virus.

Attenuated chimeric flavivirus bearing attenuated japanese encephalitis virus gene as backbone

Attenuated chimeric flavivirus bearing attenuated Japanese encephalitis virus gene as backbone

The present invention provides a novel inactivated virus particle and a reinforced immunogen which have a reinforced titer about twice to about 10 times that of a conventional vaccine, as well as a method for producing the same. The inactivated virus particle is produced by inactivating and then purifying a culture by physical means. The inactivated virus particle of the present invention is useful in a diagnostic agent for infectious disease caused by a group of Japanese encephalitis virus.

Enhanced immunogen for inactivated vaccine for infection with Japanese encephalitis viruses and process for producing the same

Growing community of inventors

Kanonji, Japan

Toyokazu Ishikawa