Takara Shuzo Co., Inc.

Takara Bio Inc.

Toyohisa Kurita

Ikunoshin Kato

Hiroyuki Izu

Makoto Ito

Nozomu Okino

Noriyuki Sueyoshi

Katsuhiro Kita

Masanori Fujita

Susumu Mitsutake

Mutsumi Sano

A process for producing lysosphingolipids by using an enzyme capable of specifically hydrolyzing the acid amide bond between a sphingoid and a fatty acid in a sphingolipid, characterized in that the enzyme reaction is carried out in a two-phase system liquid reaction mixture containing at least an organic solvent forming an aqueous phase and a separation phase in the presence of, if desired, a surfactant.

Process for producing lysosphingolipids

The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.

Sphingolipid ceramide N-deacylase, methods for producing sphingolipids and sphingolipid derivatives, and sphingolipid ceramide N-deacylase gene

Sphingolipid ceramide N-deacylase, methods for producing sphingolipids and sphingolipid derivatives, and spingolipid ceramide N-deacylase gene

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Shiga, Japan

Toyohisa Kurita