Novo Nordisk A/s

Novozymes A/s

Novo Nordisk Biotech, Inc.

Novo Nordiskals

Tove Christensen

Esper Boel

Carsten Mailand Hjort

Helle Fabricius Woldike

Ida Birgitte Huge-Jensen

Claus Crone Fuglsang

Peter Jan Punt

Frank H J Schuren

Helle Fabbicius Woldike

Torben Halkier

Sven Branner

Jeffrey R Shuster

Sven Hastrup

Jan Lehmbeck

Donna L Moyer

Charlotte Johansen

Helle Fabricius Wöldike

Mark Madden

Cees Van Den Hondel

F H J Schuren

P J Punt

C A M J J Van Den Hondel

Michael J Hynes

Birthe Ravn Jo Slashedrgensen

Birgitte Bojer Jo Slashedrgensen

Cees A M J J Van Den Hondel

Carsten M Hjort

C Mjj Van Den Hondel

The present invention relates to a transcription factor found in filamentous fungi, especially in Aspergillii, DNA sequences coding for said factor, its transformation into and expression in fungal host organisms, and the use of said factor in such hosts for increasing the expression of a polypeptide of interest being produced by said host.

Transcription factor

The present disclosure relates to isolated nucleic acid sequences encoding polypeptides having transcriptional activation activity and to the polypeptides. The disclosure also relates to nucleic acid constructs, vectors and host cells including the nucleic acid sequences. The invention further relates to host cells useful for the production of polypeptides in which the production or function of the transcriptional activator has been altered, as well as to methods for producing the polypeptides.

Fungal transcriptional activator useful in methods for producing polypeptides

The present invention relates to isolated nucleic acid sequences encoding polypeptides having transcriptional activation activity and to the polypeptides. The invention also relates to nucleic acid constructs, vectors and host cells comprising the nucleic acid sequences. The invention further relates to host cells useful for the production of polypeptides in which the production or function of the transcriptional activator has been altered, as well as to methods for producing the polypeptides.

A process for expression of a protein product inis disclosed. The process comprises transformingwith a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in. Examples of such products are chymosin or prochymosin other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinantlipase. The recombinantlipase fromdiffers from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.

Process for the production of protein products in aspergillus

Isolated transcription factor for an alpha-amylase promoter in filamentous fungi

A transcription factor found in filamentous fungi, especially in Aspergillii, DNA sequences coding for said factor, its transformation into and expression in fungal host organisms, and the use of said factor in such hosts for increasing the expression of a polypeptide of interest being produced by said host.

DNA encoding a transcription factor for an &agr;-amylase promoter in filamentous fungi

The present invention relates to a cloned DNA sequence encoding an enzyme with dextranase activity, a recombinant expression vector comprising said DNA sequence, a filamentous fungus host cell, a method for producing said recombinant dextranase, and the isolated and purified enzyme. The invention also relates to compositions comprising the recombinant enzyme, oral care compositions and products and the use for removing of dental plaque.

Recombinant enzyme with dextranase activity

Fungus wherein the areA gene has been modified and an areA gene from

The present invention relates to fungi, which do not produce proteases. The fungi of the invention are useful as hosts for the production of proteins susceptible of proteolytic degradation by the proteases usually produced, and the invention consequently encompasses processes for the production of proteins of interest in high yields by using the fungi of the invention. The invention also comprises methods for producing such fungi and DNA constructs to be used in these methods.

Fungus wherein the areA, pepC and/or pepE genes have been inactivated

A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.

Methods for producing Humicola lipases in aspergillus

A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.

Nucleic acid encoding a recombinant humicola sp. lipase

Humicola lipase produced in aspergillus

The present invention is related to a process for producing an active enzyme comprising fermenting the proform of the active enzyme in the present of a proteolytic enzyme different from the active enzyme and capable of converting the proenzyme into an active enzyme as well as to host cells, recombinant expression vectors and host cells suitable for use in the process.

Processes for producing an enzyme

Growing community of inventors

Lyngby, Denmark

Tove Christensen