Osaka University

Toshiyuki Onishi

Masahide Ishibashi

Toyokazu Ishikawa

Isao Fuke

Michiaki Takahashi

Hideo Goda

Shinichi Miyake

Takeshi Nabeshima

Kouichi Morita

Tadashi Imagawa

Morita Kouichi

Hironori Yoshii

A nucleic acid molecule containing nucleotide sequences that encode the capsid protein, pre-membrane protein and non-structural protein of Japanese encephalitis virus, and a nucleotide sequence that encodes the envelop protein of a second flavivirus, wherein the nucleotide sequence(s) that encode(s) the pre-membrane protein and/or non-structural protein of Japanese encephalitis virus contain(s) nucleotide mutations that produce one or more amino acid mutations that attenuate the virus.

Attenuated chimeric flavivirus bearing attenuated japanese encephalitis virus gene as backbone

Attenuated chimeric flavivirus bearing attenuated Japanese encephalitis virus gene as backbone

The present invention provides a novel inactivated virus particle and a reinforced immunogen which have a reinforced titer about twice to about 10 times that of a conventional vaccine, as well as a method for producing the same. The inactivated virus particle is produced by inactivating and then purifying a culture by physical means. The inactivated virus particle of the present invention is useful in a diagnostic agent for infectious disease caused by a group of Japanese encephalitis virus.

Enhanced immunogen for inactivated vaccine for infection with Japanese encephalitis viruses and process for producing the same

Growing community of inventors

Kanonji, Japan

Toshiyuki Onishi