University of Texas System

Other

Oncolys Biopharma, Inc.

Toshiyoshi Fujiwara

Noriaki Tanaka

Jack A Roth

Wei-Wei Zhang

Tapas Mukhopadhyay

Elizabeth A Grimm

Laurie B Owen-Schaub

Philippe Leboulch

Naoya Kobayashi

Satoru Kyo

Toshinori Totsugawa

Hiroyuki Mizuguchi

Takao Hayakawa

Yoshiko Shirakiya

Takeshi Kawashima

Satoru c/o Kanazawa University Kyo

By using a virus having a gene sequence comprising a telomerase promoter and an E1 gene (preferably a sequence comprising E1A gene, IRES sequence and E1B gene) or by using an anticancer agent comprising the virus, the virus replicates in cancer cells to thereby produce an efficient anticancer effect.

Oncolytic virus replicating selectively in tumor cells

The present invention provides a reagent for cancer cell detection or cancer diagnosis. The present invention relates to a reagent for cancer cell detection, comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a labeling cassette comprising a gene encoding a labeling protein and a promoter capable of regulating the expression of the gene encoding the labeling protein is integrated in E3 region of the viral genome.

Telomelysin/GFP-expressing recombinant virus

The present invention provides a mammalian immortalized liver cell obtained by transferring a cell proliferation factor gene located between a pair of site-specific recombination sequences into a mammalian liver cell.

Mammalian immortalized liver cell

The present invention relates to the use of tumor suppressor genes in combination with a DNA damaging agent or factor for use in killing cells, and in particular cancerous cells. A tumor suppressor gene, p53, was delivered via a recombinant adenovirus-mediated gene transfer both in vitro and in vivo, in combination with a chemotherapeutic agent. Treated cells underwent apoptosis with specific DNA fragmentation. Direct injection of the p53-adenovirus construct into tumors subcutaneously, followed by intraperitoneal administration of a DNA damaging agent, cisplatin, induced massive apoptotic destruction of the tumors. The invention also provides for the clinical application of a regimen combining gene replacement using replication-deficient wild-type p53 adenovirus and DNA-damaging drugs for treatment of human cancer.

Methods and compositions comprising DNA damaging agents and p53

The present invention provides a method comprising a step of transferring a cell proliferation factor gene into a mammalian liver cell to obtain an immortalized liver cell, a step of proliferating the immortalized liver cell, and a step of removing the cell proliferation factor gene from the immortalized liver cell; a large number of liver cells obtained thereby; and a treating agent and a artificial liver comprising obtained liver cell.

Method for proliferating a liver cell, a liver cell obtained thereby, and use thereof

Methods and compositions comprising DNA damaging agents and P53

The present invention provides a safe immortalized bone marrow mesenchymal stem cell obtained by transferring a cell proliferation factor gene inserted between a pair of site-specific recombination sequences to a bone marrow mesenchymal stem cell.

Immortalized bone marrow mesenchymal stem cell

Growing community of inventors

Okayama, Japan

Toshiyoshi Fujiwara