Nipro Corporation

Japan As Represented by President of National Cardiovascular Center

Japan Health Sciences Foundation

Tokyo Medical and Dental University

National Cerebral and Cardiovascular Center

Toshiya Fujisato

Akio Kishida

Takeshi Nakatani

Soichiro Kitamura

Dohiko Terada

Kazuya Sawada

Tsuyoshi Kimura

Hisatoshi Kobayashi

Seiichi Funamoto

Takuro Kogoshi

Yusuke Suga

It is intended to provide a method of preparing a decellularized soft tissue whereby swelling after an ultrahigh pressure treatment can be inhibited, etc. The above-described method of preparing a decellularized soft tissue comprises the application step wherein an ultrahigh hydrostatic pressure is applied to an isolated soft tissue in a medium to thereby disrupt cells in the soft tissue, and the removal step wherein the disrupted cells are removed. As the medium, use is made of an aqueous solution containing a water-soluble polysaccharide such as dextran.

Method of preparing decellularized soft tissue, graft and culture material

Disclosed is a method for production of a bio-derived scaffold for use in regenerative medicine, which has a bio-compatibility and biodegradability and can self-organize and grow without causing calcification. The method comprises the steps of partially fixing a biological soft tissue with glutaraldehyde by cross-linking and incubating the partially fixed tissue together with an elastase.

Method for preparing biological scaffold material

A method of injecting cells into a biological tissue comprising thrusting injection needle () under microvibration into tissue () secured by suction to tissue suction means () and effecting injection of a cell suspension into the tissue through the needle. There is also provided an apparatus for cell injection into the biological tissue.

Method of cell injection into biotissue and apparatus therefor

An apparatus and method for inoculating a biological substrate with cells include provision for rotating a container in which the substrate and a cell suspension are received about first and second axes of rotation. The apparatus comprises said container, first drive means for rotatably supporting the container about the first axis, and second drive means for rotatably supporting the first drive means about the second axis. The first and second axes of rotation do not lie in the same plane and extend in different directions so that the container revolves around the second axis while the container is rotating about the first axis. The inoculation method utilizes this apparatus.

Device and method for uniformly inoculating a substrate having three-dimensional surfaces

A method of efficiently removing or fixing donor cells from a native tissue of mammalian origin comprises immersing the tissue in a treating solution, and irradiating the tissue with microwave while maintaining the temperature thereof in the range between 0&#xb0; C. and 40&#xb0; C.

Method of treating biological tissue by microwave-irradiation

A method for preparing a scaffold material for use in the prosthesis therapy is disclosed. The method comprises (a) lyophilizing a segment of native soft tissue of mammalian origin, heating the lyophilized tissue at a temperature of 100-200&#xb0; C., and incubating the tissue with elastase to selectively remove elastin leaving the extracellular components mainly comprised of collagen.

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Suita, Japan

Toshiya Fujisato