Hitachi Chemical Company, Ltd.

Hitachi Chemical Research Center, Inc.

Toshit Sen

Cindy M Yamamoto

The present disclosure relates to the isolation of a novel reagent selected for its binding characteristics to the proteins internalin B or internalin A. InIB is a surface-localized protein ofthat binds and activates the receptor tyrosine kinase Met. InIB promotes invasion of a number of cells including hepatocytes, endothelial and epithelial cell lines and causes activation of the actin-mediated internalization of the bacterium. InIA belongs to a large group of surface-localized leucine-rich repeat (LRR) proteins identified in thegenome. InIA enablesto invade non-phagocytic cells such as those of the human intestinal epithelium and is sufficient for adhesion to and inducing uptake into epithelial cells. The disclosed nucleic acid ligands to internalin B and internalin A may be useful for determining the presence or absence of internalin B, internalin A, orin food, clinical or environmental samples; they may also be useful as an agent for combatinginfection by binding to and inactivating the infection-promoting inlB or inlA proteins. One object is to incorporate these nucleic acid ligands into an in vitro diagnostic or biosensor platform designed to detect the presence or absence of internalin B, internalin A, orin food, clinical or environmental samples. Another object is to employ these nucleic acid ligands in methods for treating or preventinginfection.

Nucleic acid ligands capable of binding to internalin B or internalin A

Aptamers bind tosurface proteins. A method of assaying a sample for the presence ofincludes exposing the sample to an aptamer that specifically binds one of the following proteins:internalin A protein,internalin E protein, and0610 protein. The presence ofin the sample is detected when the aptamer binds the protein present in the sample. A method of treatinginfection includes administering the aptamers to the mammal at a concentration sufficient to reduceinfection.

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Tustin, CA, United States of America

Toshit Sen