Kyoto Prefectural Public University Corporation

Ohgi Technological Creation Co., Ltd.

Toshiro Yamamoto

Osam Mazda

Tsunao Kishida

Kenta Yamamoto

Yoshihiro Sowa

Ken-ichi Honjo

Takehiko Oki

Tatsuhiko Oki

Narisato Kanamura

Provided is a method of preparing a somatic cell including converting a differentiated somatic cell of a mammal to other somatic cell by culturing the differentiated somatic cell in a medium for inducing differentiation of the somatic cell other than the differentiated somatic cell in the presence of a TGF-β pathway inhibitor.

Cell preparation method

An object of the present invention is to provide a method for preparing osteoblasts that are applicable, without causing risk of canceration, to bone defect repair or to the treatment of bone resorption, fracture, osteoporosis, or the like. To solve this problem, the present invention provides a method for preparing osteoblasts, the method comprising culturing mammal differentiated somatic cells in a medium in the presence of at least one compound selected from the group consisting of (1) statin compounds, (2) casein kinase 1 inhibitors, (3) cAMP inducers, and (4) histone methyltransferase inhibitors, to convert the somatic cells into osteoblasts.

Method for preparing osteoblasts and osteoblast inducer

An object of the present invention is to provide a method for generating an osteoblast that is applicable to repair of a bone defect due to various tumors, injuries, surgeries, etc. and to treatment for bone resorption typified by a periodontal disease, bone fracture, osteoporosis, etc., and that has a low risk of carcinogenesis. Provided as a means for achieving this object is a method for generating an osteoblast from a somatic cell of a mammal, the method comprising introducing Oct9 gene or an expression product thereof into the somatic cell.

Osteoblasts and method for generating same

The present invention relates to: a method of preparing an osteoblast from a somatic cell of a mammal, the method including introducing a bone-related gene or an expression product thereof and a reprogramming-related gene or an expression product thereof, or introducing a reprogramming-related gene or an expression product thereof independently into the somatic cell, the bone-related gene including at least one kind selected from the group consisting of Runx2 (R), Osterix (O), and Dlx5 (D), the reprogramming-related gene including at least one kind selected from the group consisting of Oct family, c-Myc (M), L-Myc (L), Klf family, Lin-28, and Sox2; and an osteoblast prepared by the method.

Osteoblast and method for preparing same

A gum massaging tool includes a handle portion which accommodates electric or mechanical members and in which a selector switch is provided in an exposed manner; a fixed shaft portion that is fixed to the handle portion so as to extend from one end of the handle portion and is heated according to an operation of the selector switch; a gum contact portion that is rotatable around a distal end portion of the fixed shaft portion and that includes a carbon body which receives heat from the fixed shaft portion and transfers the heat to a gum contacting surface; and a supporting portion that is rotatably engaged with and supports the gum contact portion and is detachably attached to the handle portion.

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Kyoto, Japan

Toshiro Yamamoto