Toyo Boseki Kabushiki Kaisha

Toshiro Kikuchi

Shigenori Emi

Kazumi Yamamoto

Masachika Takashio

Minoru Kamimura

Makoto Ando

Yoshio Kojima

Kohji Sugihara

Tatsuro Shigyo

Yuji Takamoto

Masanori Oka

Masaru Ogawa

This invention relates to DNA containing a gene encoding uricase, a plasmid having said DNA, a transformant containing said plasmid, and a process for producing uricase by using said transformant.

DNA sequence for uricase and manufacturing process of uricase

This invention relates to an isolated uricase which is stable in an aqueous solution at a temperature up to 60.degree. C. and at a pH of 8.0 for 10 minutes.

Isolated recombinant uricase

A DNA fragment that contains a gene encoding creatinine amidohydrolase is provided. This invention also provides a recombinant vector containing the said DNA fragment, a transformant containing the said vector, and a method for producing creatinine amidohydrolase by the use of the said transformant.

DNA fragment containing a gene encoding creatinine amidohydrolase

A pyruvate oxidase with excellent thermal stability, which is stable at pH 7.0 for 10 minutes at a temperature of up to 45.degree. C., an analytical method using said pyruvate oxidase, and an analytical reagent used for said method.

Pyruvate oxidase and an analytical method using the same

Novel acyl-coenzyme A oxidase is produced by cultivating an acyl-coenzyme A oxidase-producing microorganism belonging to the genus Candida in a nutrient medium, thereby accumulating an amount of acyl-coenzyme A oxidase in yeast cells and recovering the acyl-coenzyme A oxidase from said cells.

Process for producing acyl-coenzyme A oxidase

There is provided a method for the quantitative analysis of a free fatty acid which comprises (1) the first step of treating a sample containing the free fatty acid with acyl-coenzyme A synthetase in the presence of adenosine triphosphate and coenzyme A to form acyl-coenzyme A, (2) the second step of oxidizing said acyl-coenzyme A, in the presence of oxygen, with acyl-coenzyme A oxidase produced by a microorganism of the genus Candida to form enoyl-coenzyme A and hydrogen peroxide, and (3) the third step of (a) measuring the amount of the formed enoyl-coenzyme A or hydrogen peroxide or (b) measuring the amount of oxygen consumed in said oxidation reaction, to thereby determine the amount of free fatty acid in said sample.

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Tsuruga, Japan

Toshiro Kikuchi