Seikagaku Corporation

Seikagaku Kogyo Kabushiki Kaisha

Seikagaku Kogyo Kabushiki Kaisha (seikagaku Corporation)

Nec Corporation

Tohoku University

Toshio Oda

Hikaru Mizumura

Jun Aketagawa

Shun-ichiro Kawabata

Hiroshi Tamura

Shigenori Tanaka

Sadaaki Iwanaga

Maki Aizawa

Tatsushi Muta

Noriaki Seki

Yuki Kobayashi

Tomokazu Matsue

Kumi Inoue

Shun-Ichiro Kawabata

Provided is a technology related to a horseshoe crab factor B variant, and also provided is means for performing endotoxin measurement with high sensitivity. A polypeptide having an amino acid sequence in which the amino acid residue at the 193-position in an amino acid sequence of a polypeptide of horseshoe crab factor B is substituted with a cysteine (Cys) residue, is produced. Endotoxin measurement can be carried out with high sensitivity by configuring areagent by combining this polypeptide with horseshoe crab factor C.

Horseshoe crab factor B variant

A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from, and, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.

Recombinant Factor C and method for producing the same, and method for measuring endotoxin

A method for rapidly and highly sensitively measuring endotoxin relies on an endotoxin-measuring agent, which includes a factor C derived fromthat does not have His-tag sequence at the C-terminus, a factor B of a horseshoe crab, and a proclotting enzyme of a horseshoe crab. Each of these proteins can be a recombinant protein obtainable by being expressed using a stably expressing cell line of an insect cell as a host.

Method for producing factor C recombinant protein and endotoxin-measuring agent

To provide a method for producing a horseshoe crab recombinant Factor C. The horseshoe crab recombinant Factor C is produced through expression thereof by use of mammalian cells such as CHO DG44 and HEK293 as host cells.

Recombinant factor C and method for producing the same, and method for measuring endotoxin

The present invention addresses the problem of providing a means for making highly sensitive and stable measurements possible using electrochemical measurement methods. This problem is resolved by providing: a labeling substance represented by general formula (1) and used to label a substrate; a measurement substrate that is formed by being labeled using the labeling substance; a method of measuring using electrochemical measurement methods that use the measurement substrate; and a reagent kit that includes as components the labeling substance and/or the measurement substrate. (In general formula (1), Ris either H or an alkyl group (CH), m is an integer of 1-4, Ris an alkyl group (CH), and n is an integer of 1-4.)

Electrochemical measurement using phenylenediamine derivative

To provide means for reducing reaction interference observed when AT III is subjected totest, whereby thetest of antithrombin III can be carried out at high accuracy. Reaction interference observed when AT III is subjected totest can be reduced by subjecting AT III to a protein inactivation treatment in the co-presence of a divalent metal salt.

Pre-treatment agent and pre-treatment method for antithrombin III to be subjected to limulus test

A pretreatment agent for a sample to be subjected toassay comprising an alkali metal sulfate and/or an alkaline earth metal sulfate wherein the sulfate(s) has a final concentration of 20 mM or more when the sulfate(s) is allowed to contact with the sample, or an alkali metal halide and/or an alkaline earth metal halide wherein the halide(s) has a final concentration of from 0.4 M to 1.2 M or less when the halide(s) is allowed to contact with the sample, or a kit forassay reagent comprising thereof as a composing article.

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In a data communication system with an automatic repeat request (ARQ) protocol, a method/system capable of setting an optimum value of the RTF, which is used for threshold value of waiting time from transmitting a certain frame until retransmitting the same frame due to no acknowledgement during the waiting time, is provided. During the operation for establishing synchronization, in which a frame for control signal is transmitted repeatedly until receiving acknowledgement from the other end of apparatus, between arbitrary two apparatuses, the transmission delay is detected by using a forward count control information and backward count control information which are newly added in the forward channel information field of the frame format. The forward count control information is incremented by one for every repeated frame, and when the receiving apparatus has received one of the repeated frames, the value of the forward count control information is copied to the backward count control information in a frame to be returned. At the transmission apparatus, when the acknowledgement frame has been received, transmission delay is calculated by subtracting the value of the backward count control information in the received frame from the value of the forward count control information for a frame having been transmitted just before. This subtracted value is used as the RTF.

Method and system for automatic setting of optimum number of waiting

This invention relates to a DNA shown by SEQ ID No. 1 and an amino acid sequence coded by said DNA. This invention also relates to a DNA and an amino acid sequence of subunit a of (1.fwdarw.3)-.beta.-glucan sensitive factor derived from amebocytes of horseshoe crab and have potent affinity to (1.fwdarw.3)-.beta.-D-glucan in cell walls of fungi. Therefore, the invention is useful for the diagnosis of fungal diseases and as antimicrobial or eradicating agent of fungi in combination with an antifungal agent.

DNA encoding horseshoe crab amebocyte lysate factor G subunit A

An endotoxin stabilizing agent is provided, which is useful in order to maintain the endotoxin activity of a specimen or a reference standard in a stable state for a prolonged period of time and to prepare an endotoxin reference standard having a reduced intervial variation, being stable for a long time in the form of, in particular, a solution and withstanding repeated use. An endotoxin composition comprising the above-mentioned endotoxin stabilizing agent and endotoxin and a method for assaying endotoxin by using the same are also provided. The endotoxin stabilizing agent comprises: (1) at least one substance selected from the group consisting of aminoalcohols, polyhydric alcohols, nonionic surfactants, polysucroses and chelating agents; (2) at least aminoalcohol and polyhydric alcohol; (3) with the polyhydric alcohol being preferably glycerol or its derivative; (4) polyethylene glycol as an effective ingredient in addition to the components (1) to (3); or (5) at least polysucrose, and an alkaline earth metal salt and/or polyethylene glycol as an effective ingredient. The endotoxin composition comprises the above-identified endotoxin stabilizing agent. The method for assaying endotoxin comprises adding the above-described endotoxin stabilizing agent to a specimen to thereby stabilize the activity of endotoxin in the specimen.

Endotoxin stabilizing agent, endotoxin composition and method for

Horseshoe crab amebocyte lysate factor G subunit A

Endotoxin (Et) can be specifically assayed by exclusively utilizing the factor C system reaction without being affected by factor G contained in a limulus amebocyte lysate reagent. The present invention provides: (1) a reagent for ET-specific assay which comprises a limulus amebocyte lysate reagent and an alkylglucoside; (2) a method of specifically assaying Et in a specimen using a limulus amebocyte lysate reagent, wherein an alkylglucoside is added to the limulus amebocyte lysate reagent and/or the specimen; and (3) a factor G activation inhibitor composition which comprises an alkylglucoside as an active ingredient capable of inhibiting the activation of factor G in limulus amebocyte by (1.fwdarw.3)-.beta.-D-glucan.

Growing community of inventors

Tokyo, Japan

Toshio Oda