Ciba Geigy Japan Limited

Japat Limited

Toshio Kokubo

Tetsuya Inaoka

Toyomi Ohkuma-Soyejima

Tadashi Yoshimoto

Daisuke Tsuru

The present invention relates to the field of biotechnology and concerns heat-stable prolylendopeptidase, recombinant DNA coding for heat-stable prolylendopeptidase, and processes for the production of heat-stable prolylendopeptidase and of recombinant DNA coding therefor, a host transformed with said recombinant DNA and a process for the production of said transformed host.

Heat-stable prolylendopeptidase

The invention concerns DNA encoding prolylendopeptidase, hybrid vectors containing such DNA, transformed hosts capable of expressing prolylendopeptidase, a process for the production of prolylendopeptidase including the steps of: culturing a host organism transformed with an expression vector including a DNA coding for prolylendopeptidase and optionally, recovering the produced prolylendopeptidase; and a process for the production of a C-terminal amidated peptide from two precursors, including the steps of: placing the two precursors in contact with a prolylendopeptidase in a medium to convert the precursor peptides to the C-terminal amidated peptide, and recovering the resulting C-terminal amidated peptide.

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Sanda, Japan

Toshio Kokubo