Juridical Foundation the Chemo-sero-therapeutic Research Institute

Teijin Limited

Toshinobu Nouchi

Hiroshi Mizokami

Yoshinobu Miyatsu

Yoshitaka Tajima

Kiyoto Nishiyama

Keishin Sugawara

Shinji Nakahira

Satoshi Adachi

Yoichi Ogata

Yoshitaka Ushio

Masahiro Sakaguchi

Kazunari Yagi

A method for producing influenza virus on large scale is provided. A method for propagating influenza virus which comprises, after removing or decreasing a trypsin inhibitor secreted into culture of MDCK cells (cell line derived from dog kidney) by washing with a culture medium or a buffer, inoculating influenza virus into said cells and culturing said influenza virus-inoculated cells in a culture medium supplemented with trypsin.

Method for propagating influenza virus

Multimers of human serum albumin are removed from a solution of human serum albumin containing the multimers by bringing the human serum albumin solution into contact with an anion-exchanger equilibrated with a buffer containing a salt in a concentration ranging from 10 to 150 mM and having a pH value ranging from 5 to 9.5.

Method for removing multimers of human serum albumin

A method for preparing human serum albumin comprises the step of heat-treating a human serum albumin solution containing contaminants at a pH value in the proximity to the isoelectric point of the contaminants.

Method for preparing human serum albumin containing heat-treatment

Human activated protein C and process for preparing same

Growing community of inventors

Kumamoto, Japan

Toshinobu Nouchi