Sysmex Corporation

Other

Toshihiro Mizukami

Tomohiro Tsuji

Yusuke Mori

Tatsuya Narikawa

Takashi Sakata

Shinichiro Oguni

Aya Konishi

Hiroki Takeshita

Yukie Nakazawa

Toshifumi Takeuchi

Takaaki Nagai

Noriyuki Nakanishi

Konobu Kimura

Yoshiro Ikeuchi

Ayumu Yoshida

Hideki Hirayama

Yuji Itose

Munetaka Ishiyama

Ken Nishikawa

Keiko Moriyama

Yuji Toya

Kayo Hatanaka

Saori Suzuki

Kanako Horisaka

Jun Matsui

Yuuichi Hamada

Masato Kuze

Noriaki Nakajima

Hironori Tanaka

Saori Otsuka

Kazuki Hatcho

Sunao Atogami

Daigo Fukuma

Seiichiro Tsuchiya

Disclosed is a measurement apparatus for analyzing a cell contained in a specimen, comprising: a chamber for preparing a measurement sample in which the cell is stained with first and second fluorescent dyes contained in a reagent supplied from at least one reagent container; a liquid feeding section for feeding the reagent from the reagent container to the chamber via a liquid feeding tube provided between the reagent container and the chamber; and a detection section that acquires first and second signals each corresponding to fluorescence of a first wavelength and fluorescence of a second wavelength emitted from the cell stained with the first and second fluorescent dyes in response to irradiation of the measurement sample flowing in a flow cell with light; and an analysis section that analyzes the cell on the basis of the first and second signals.

Measurement apparatus and analysis method

The present invention provides a method for classifying and counting leukocytes which allows classification and count of normal leukocytes as well as discrimination between blast cells and atypical lymphocytes. The present invention also provides a regent kit and reagent for classifying leukocyte which are used for classifying and counting leukocytes in biological samples.

Method for classifying/counting leukocytes, reagent kit for classifying leukocytes, and reagent for classifying leukocytes

Disclosed is a method of detecting filarial larvae in blood. The method comprises preparing a measurement sample from a blood sample collected from an animal; flowing the prepared measurement sample through a flow cell; irradiating light on the measurement sample flowing through the flow cell; detecting light given off from the irradiated measurement sample; and detecting filarial larvae contained in the measurement sample based on characteristic parameter of the detected light.

Method of detecting filarial larvae in blood

The present invention provides a reagent kit for analyzing a sample comprising a first reagent containing a cationic surfactant, a nonionic surfactant and an aromatic carboxylic acid and a second reagent containing a fluorescent dye capable of staining nucleic acid, and a method for analyzing a sample using the kit.

Reagent kit for sample analysis and sample analysis method

A reagent for measuring basophils and/or nucleated red blood cells in a sample, which comprises at least one fluorescent dye defined in the formula (I) or (II) is disclosed. Also disclosed are a kit and method for measuring basophils and/or nucleated red blood cells.

Reagent for sample analysis, kit for sample analysis and method for sample analysis

A reagent for measuring a basophil and/or a nucleated red blood cell is described. The reagent comprises (a) an alcohol having 4 to 8 total carbon atoms, and (b) one or more fluorescent dyes selected from the group consisting of a compound of the general formula (I) and a compound of the general formula (II).

Reagent for sample analysis, reagent kit for sample analysis and method for sample analysis

A method for classifying and counting leukocytes, which comprises steps of: staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent, with a fluorescent dye; introducing the sample containing the stained cells into a flow cytometer to measure first scattered light, second scattered light different from the first scattered light and fluorescence of the respective cells; obtaining scattered light peak intensities and scattered light widths of the respective cells based on the measured first scattered light, obtaining scattered light intensities of the respective cells based on the measured second scattered light, and obtaining fluorescence intensities of the respective cells based on the measured fluorescence light; classifying the cells into a first group and a second group based on the scattered light peak intensities and the scattered light widths, the first group including leukocytes and second group including platelet clumps; classifying the leukocytes included in the first group into at least lymphocytes, monocytes and granulocytes based on the scattered light intensities and the fluorescence intensities; and counting the classified lymphocytes, the classified monocytes and the classified granulocytes.

Method of classifying and counting leukocytes

There is provided a method for classifying and counting leukocytes with abnormal DNA amount, which comprises:

Method for classifying and counting leukocytes

A method for counting megakaryocytes in a specimen is described. In the method, first, erythrocytes in the specimen are lysed and nucleic acid in the megakaryocytes is stained with a fluorescent dye, and thereby, a measurement sample is prepared. Next, the cells in the measurement sample are irradiated with excited light so that the forward scattered light, the side scattered light and the fluorescence, which are emitted from the cells, are detected. Megakaryocytes are identified on the basis of the detected forward scattered light, the fluorescence and the side scattered light. Then, the identified megakaryocytes are counted.

Method for counting megakaryocytes

Methods and devices are described for monitoring a therapeutic effect in a red blood cell-related disease by detecting the size of a reticulocyte using a full-automatic blood cell counting device.

Methods and devices for measuring reticulocytes

A method for evaluating an artificial receptor includes subjecting a functional monomer and a crosslinker in the presence of a template substance in a vessel into polymerization, thereby forming a resultant polymer with the template substance usually in the form of a thin film, dissociating the template substance from the polymer and removing it from the vessel by washing an inside of the vessel, thereby to prepare an artificial receptor, feeding a solution of the same or different template substance into the vessel to associate the template substance with the resulting polymer; then determining the amount of the template substance remaining in the vessel, and evaluating the artificial receptor on the basis of a determination result.

Method for evaluating artificial receptors

A reagent for classification and counting of leukocytes, the reagent containing at least one dye which has the following structural formula ##STR1## where R.sub.1 is a hydrogen atom or an alkyl group, R.sub.2 and R.sub.3 each represent a hydrogen atom, a lower alkyl group or a lower alkoxy group, R.sub.4 represents a hydrogen atom, an acyl group or an alkyl group, Z represents a sulfur atom, an oxygen atom, or a carbon atom having a lower alkyl group, n denotes 0, 1 or 2, and X.sup.- represents an anion, and specifically binds to RNA to increase in fluorescence intensity, and a method for classification and counting of leukocytes which uses the reagent, whereby abnormal cells such as immature leukocytes and abnormal leukocytes can be classified and counted easily and highly accurately, and at the same time, classification and counting of normal leukocytes as well as the counting of leukocytes can be performed.

Reagent and method for classification and counting of leukocytes

A hematological sample is treated with a hemolytic agent that maintains immature leukocytes in a viable state and damages other leukocytes. The damaged leukocytes are stained with a fluorochrome which can stain damaged cells. At least one kind of scattered light and at least one kind of fluorescence of the treated and stained blood cells are measured to classify and count leukocytes based on the intensities of the scattered light and the fluorescence. Highly precise measurement of immature leukocytes and simultaneous classification and counting of normal leukocytes are achieved.

Growing community of inventors

Kobe, Japan

Toshihiro Mizukami