Toyo Boseki Kabushiki Kaisha

Cellfree Sciences Co., Ltd.

Toshihiro Kuroita

Yoshihisa Kawamura

Bunsei Kawakami

Yoshiaki Nishiya

Masao Kitabayashi

Tadayuki Imanaka

Hideyuki Komatsubara

Yoshikazu Ishida

Atsushi Sogabe

Yaeta Endo

Yutaka Takarada

Masanori Oka

Shigemichi Nishikawa

Hideki Kamimura

Naoki Tanaka

It is intended to provide a method of conveniently screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; and a protein achieving an improved blocking efficiency that can be expressed on a large scale in. A method of screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; a protein characterized by achieving an improved blocking efficiency owing to an amino acid sequence modification; and a method of utilizing the protein.

Protein achieving improved blocking efficiency

An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DXEXXXH sequence (D: aspartic acid, E: glutamic acid, H: histidine, X, X, Xand X: any amino acid) consisting of DXE sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3'-5&#x2032; exonuclease activity, histidine(H) has been replaced by another amino acid.

Modified thermostable DNA polymerase

The invention provides methods, kits, and compositions for enhancing synthesis of DNA involving a carboxylate ion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions. The invention further provides a thermostable DNA polymerase-related factor derived fromspecies, which has an activity to promote the DNA synthesis activity of DNA polymerase or which binds to DNA polymerase.

Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof

The present invention provides a composition for cell-free protein synthesis, which is superior in storage stability in a freeze-dried state, more particularly a freeze-dryable or freeze-dried composition for cell-free protein synthesis, which contains a cell extract for cell-free protein synthesis and inositol, and a freeze-dryable or freeze-dried composition for cell-free protein synthesis containing a cell extract for cell-free protein synthesis, and a deliquescent material in a proportion of not more than 0.01 part by weight per part by weight of a protein in the composition; and a composition for cell-free protein synthesis superior in storage stability in a frozen state, more particularly a freezable or frozen composition for cell-free protein synthesis, containing a cell extract for cell-free protein synthesis and polyhydric alcohol.

Composition for cell-free protein synthesis

A method for isolating a ribonucleic acid, which comprises dissolution of a sample containing the ribonucleic acid, such as cells, in an acidic solution containing a lithium salt and a chaotropic agent, bringing the ribonucleic acid into contact with a nucleic acid-binding carrier such as silica particles, thereby to allow selective adsorption of the ribonucleic acid alone onto said carrier, and eluting the ribonucleic acid from the nucleic acid-bound carrier; a reagent therefor; and a method for producing a cDNA from the ribonucleic acid isolated by this method. According to the present invention, a high purity ribonucleic acid can be isolated quickly and safely from a sample containing the ribonucleic acid.

Method for isolating ribonucleic acid

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Tsuruga, Japan

Toshihiro Kuroita