Adeka Corporation

Niigata University

Toshihiko Takehana

Seiji Koike

Tsutomu Sato

Koichi Shigeno

Tomohisa Kuzuyama

Tsutomu Hoshino

Kotone Okuno

An object of the present invention is to provide a method for preparing ambrein, which can easily and efficiently obtain the ambrein. The object can be solved by a mutated tetraprenyl-&#x3b2;-curcumene cyclase wherein

Efficient method for producing ambrein

An object of the present invention is to provide a method for preparing ambrein, which can easily obtain the ambrein. The object can be solved by a mutated tetraprenyl-&#x3b2;-curcumene cyclase wherein a fourth amino acid residue of a DXDD motif, aspartic acid, is substituted with an amino acid other than aspartic acid, (a) having a QXXXGX(W/F) motif, and a QXXXX(G/A)X(F/W/Y) motif on the N-terminal side, and a QXXXGX(F/W/Y) motif, and a QXXXGXW motif and a QXXXGX(F/W) motif on the C-terminal side, and not having a QXXXGXW motif at a position separated by 170 amino acid residues or more on the C-terminal side, with respect to the DXDD motif, (b) having 40% or more identity with the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 13, (c) exhibiting ambrein production activity using squalene as a substrate.

Variant type tetraprenyl-&#x3b2;-curcumene cyclase and method for producing ambrein

The object of the present invention is to efficiently produce useful terpenoid compounds, and specifically, to provide a method for preparing squalene, which is an important intermediate of terpenoid. The object can be solved by a hydroxymethylglutaryl CoA reductase (HMGR) comprising: (a) an amino acid other than alanine (A) at the 10th position in an S&#x3b1;2 amino acid sequence of HMGR, (b) an amino acid other than proline (P) at the 1st position from the carboxyl terminal in the DKK region of the HMG-CoA binding site of HMGR, (c) an amino acid other than alanine (A) at the 1st position in an L&#x3b1;2 amino acid sequence of HMGR, and (d) an amino acid other than glutamic acid (E) at the 6th position in an L&#x3b1;2 amino acid sequence of HMGR of the present invention.

Mutant enzyme and production method for terpenoid using said mutant enzyme

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Tokyo, Japan

Toshihiko Takehana