Japan Tobacco Inc.

E.i. Dupont De Nemours and Company

Pioneer Hi-bred International, Inc.

Kaneka Corporation

Pioneer Hi-bred Int'l Inc.

Toshihiko Komari

Yukoh Hiei

Yoshimitsu Takakura

Toshiyuki Komori

Yuji Ishida

Satoru Usami

Masakazu Kashihara

Teruyuki Imayama

Marc C Albertsen

Hajime Sakai

Stanley Luck

Scott V Tingey

Ichiro Oka

Norio Kato

Toshiki Mine

Hideaki Saito

Yoshiki Kuraya

Tomoaki Kubo

Masahiko Maekawa

Yokoh Hiei

Yasuhito Saito

The present invention relates to a method of obtaining a transformed plant cell. The present invention comprises the steps of:

Method of obtaining transformed plant cell

An object of the present invention is to provide nucleic acids capable of imparting high-yielding ability to plants. Another object of the present invention is to use such nucleic acids to produce transgenic plants at increased yield, as well as to provide methods for increasing the yield of plants. By introducing into a plant a construct in which a promoter of a pseudo-response regulator gene inand/or a structural gene of a pseudo-response regulator in a plant are operably linked, a transgenic plant is obtained that has acquired high-yielding ability.

Nucleic acid imparting high-yielding property to plant, method for producing transgenic plant with increased yield, and method for increasing plant yield

Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.

Drought tolerant plants and related constructs and methods involving genes encoding DTP21 polypeptides

The present invention aims to provide novel vectors for plant transformation. The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that:

Cosmid vector for plant transformation and use thereof

The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement. The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.

Method for screening genomic DNA fragments

Disclosed is a method of transforming monocotyledons which necessitates only a short period from the transformation to the regeneration of a whole plant as compared with the conventional methods, thus reducing the frequency of occurrence of mutants, and can be generally applied to the plants for which any system of regenerating the whole plants from protoplasts has not been established, and in which the material to be used can be readily prepared without any particular apparatuses. The present invention provides a method for transforming monocotyledons comprising transforming scutellum of an immature embryo of a monocotyledon with a bacterium belonging to genuscontaining a desired gene, which immature embryo has not been subjected to a dedifferentiation treatment, to obtain a transformant.

Method of transforming monocotyledons using scutella of immature embryos

Vectors for transforming plants with the use ofwhich have been modified so as to elevate the possibility of the recognition of the border sequences of the vectors by vir proteins of the, thereby lowering the possibility of the transfer of DNAs other than T-DNA into plant chromosomes. More particularly, the above-vectors are those to be used in transforming plants which have right and left border sequences which can be recognized by the vir proteins of the, a T-DNA sequence which is located between these border sequences and into which a gene to be transferred into plants can be inserted, and a replication origin enabling the replication of the vectors in bacteria, characterized by having a plural number of left border sequences.

Vectors for transforming plants

The invention relates to a method for transforming a monocotyledonous plant. The time required from transformation to regeneration of a plant is shorter using the inventive method so that the frequency of emergence of mutants is smaller than the conventional methods. The inventive method may be generally applied even to the plants for which a regeneration method from a protoplast to a plant has not been established, and with which the preparation of the material to be subjected to the method is easy. That is, the present invention provides a method for transforming a monocotyledonous plant, comprising contacting a cultured tissue of said monocotyledonous plant during dedifferentiation thereof obtained by culturing an explant on a dedifferentiation-inducing medium for less than 7 days with a bacterium belonging to the genuscontaining a super binary vector having the virulence region of a Ti plasmid, left and right border sequences of T-DNA of a Ti plasmid or an Ri plasmid of a bacterium belonging to the genus, and a desired gene located between said left and right border sequences.

Method for transforming monocotyledons

Method for introducing two T-DNAS into plants and vectors therefor

Growing community of inventors

Tokyo, Japan

Toshihiko Komari