Rikagaku Kenkyusho

Toshiaki Kudo

Koki Horikoshi

Chiaki Kato

Hiroshi Honda

Tetsuo Kobayashi

The present invention provides for a plasmid, pEAP7.DELTA.P, containing a DNA region capable of inducing extracellular secretion of a useful, physiologically active substance in transformed host and a promoter DNA region regulating expression of the first DNA region. The present invention further provides for a microorganism transformed with the plasmid and a process for the production of the substances by culturing the microorganism.

Secretion plasmid comprising the kilgene

A novel plasmid pEAP2, which was constructed from Bacillus sp. 170 chromosomal DNA carrying the gene for extracellular production of penicillinase and a vector plasmid pMB9. A novel microorganism, Escherichia coli HB101(pEAP2) carrying the plasmid pEAP2 and being capable of extracellular production of penicillinase. The method for cultivation of the microorganism is characterized by culturing it in the medium containing NaCl (or KCl) for 16-48 hours. According to this invention, useful high-molecular substances can be produced in a high yield.

Plasmid, method for construction of the same, microorganisms carrying

A novel plasmid pCX311, which was constructed from Bacillus sp. C125 chromosomal DNA carrying the gene for extracellular production of xylanase and a vector plasmid pBR322. A novel microorganism, Escherichia coli HB101 (pCX311) carrying the plasmid pCX311 and being capable of extracellular production of xylanase. Method for culturing the microorganism characterized by cultivation of it in the medium containing NaCl (or KCl) and bran, or NaCl (or KCl) and xylan for 12-48 hours. According to this invention, useful high-molecular substances can be produced in a high yield.

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Tokyo, Japan

Toshiaki Kudo