Perkinelmer Cellular Technologies Germany Gmbh

Evotec Technologies Gmbh

Fraunhofer-gesellschaft Zur Foerderung Der Angewandten Forschung E.v.

Jpk Instruments Ag

Other

Institut Pasteur

Evotec Biosystems Ag

Perkinelmer Cellular Technologies

Torsten Müller

Thomas Schnelle

Rolf Hagedorn

Gunter Fuhr

Bernd Wagner

Detlef Knebel

Torsten Jähnke

Kathryn Anne Poole

Günter R Fuhr

Stefan Hummel

Annette Pfennig

Stephen Shirley

Ulrich Hofmann

Thomas Lisec

Martin Stelzle

Wolfgang Benecke

Andreas Voigt

Steffen Howitz

Gabriele Gradl

Jan Gimsa

Spencer Shorte

Christoph Reichle

Jan Hornung

Martin Reichel

Hermine Hitzler

Karl-Otto Greulich

Shamoi Monajembashi

Jorg Kentsch

Frank Grom

Jörg Kentsch

Methods and devices for the separation of particles () in a compartment () of a fluidic microsystem () are described, in which the movement of a liquid () in which particles () are suspended with a predetermined direction of flow through the compartment (), and the generation of a deflecting potential in which at least a part of the particles () is moved relative to the liquid in a direction of deflection are envisaged, whereby further at least one focusing potential is generated, so that at least a part of the particles is moved opposite to the direction of deflection relative to the liquid by dielectrophoresis under the effect of high-frequency electrical fields, and guiding of particles with different electrical, magnetic or geometric properties into different flow areas () in the liquid takes place.

Methods and devices for separating particles in a liquid flow

The invention relates to a method for the combined analysis of a sample with objects to be analyzed, in particular a sample with biological objects, in which measurement results for one or more of the objects to be analyzed in the sample are obtained by analyzing the one or more objects to be analyzed by an imaging method of measurement, probe-microscopic measurement results are obtained for the one or more objects to be analyzed by analyzing the one or more objects to be analyzed by a probe-microscopic method of measurement, and the measurement results and the probe-microscopic measurement results are assigned to one another, after optional prior intermediate processing. Furthermore, the invention relates to an apparatus for carrying out combined analysis of a sample with objects to be investigated, in particular a sample with biological objects.

Method and apparatus for the combined analysis of a sample with objects to be analyzed

The invention relates to a measuring probe device for a probe microscope, in particular a scanning probe microscope, with a measuring probe holder and a measuring probe arranged on the measuring probe holder, which is set up for a probe microscopic investigation of a sample, wherein on the measuring probe holder, a measuring probe chamber is formed, which receives the measuring probe at least partially and is open on a side away from the measuring probe holder, and is configured to receive a liquid surrounding the measuring probe. The invention also relates to a measuring cell for receiving a liquid sample for a probe microscope, a scanning probe microscope with a measuring probe device and a scanning probe microscope with a measuring cell.

Measuring probe device for a probe microscope, measuring cell and scanning probe microscope

The invention relates to an operating method for a microfluidic system, including the following steps: feeding of a carrier flow with particles () of a first particle type suspended therein into the microfluidic system; charging of a plurality of electrical field cages () in the microfluidic system with the supplied particles () of the first particle type; the supplying of a carrier flow with particles () of a second particle type suspended therein into the microfluidic system; and charging the field cages () in the microfluidic system with the supplied particles () of the second particle type in such a manner that a particle () of the first particle type and a particle () of the second particle type is present in at least one of the field cages (). The invention also relates to a corresponding microfluidic system.

Microfluidic system and corresponding operating method

The present invention relates to a method for providing a measuring probe () for a probe microscopic examination of a sample in a probe microscope, in particular a scanning probe microscope, in which the measuring probe (), which has a probe base () and a probe extension () formed thereon, is held on a carrier device and the measuring probe () is processed before or after a measurement by detaching a section of the probe extension (). The invention further relates to an arrangement having a probe microscope for the probe microscopic examination of a sample, in particular a scanning probe microscope.

Method for providing a probe for a probe-microscopic analysis of a test sample in a probe microscope and arrangement with a probe microscope

A description is given of methods for collecting particles () which are suspended in a liquid, including the following steps: providing the liquid containing the suspended particles () in a compartment () having lateral surfaces (), wherein at least one electrode () is arranged on at least one of the lateral surfaces (), and generating high-frequency electric fields by means of the at least one electrode () so as to form at least one circulating flow (), by means of which the particles () are guided to at least one predetermined collecting area () in the compartment (), wherein the flow () is formed in such a way that at least one branch of the flow runs along a longitudinal extent of the at least one electrode (), and the flow () circulates about an axis () which is oriented perpendicular to the respectively adjacent lateral surface () with the electrode (). Corresponding devices for collecting particles are also described.

Method and device for collecting suspended particles

A method for high-resolution image recording of at least one object with a microscope, includes the steps of: (a) positioning the object in a receptacle being arranged in the optical axis of the microscope, (b) generating at least two first data sets per object which represent intermediate images of the object with at least two different orientations relative to the optical axis of the microscope, wherein the different orientations of the object are provided by moving the object relative to the receptacle, and (c) evaluating the data sets for obtaining quantitative three dimensional information.

Method and device for 3 dimensional imaging of suspended micro-objects providing high-resolution microscopy

The invention relates to a particle injector for introducing particles into a carrier flow of a microfluidic system, especially for injecting biological cells into the carrier flow of a cell sorter. The particle injector includes an inlet for receiving the carrier flow, an outlet for discharging the carrier flow including the introduced particles, a carrier flow channel which connects the inlet to the outlet, and an injection channel flowing into the carrier flow channel for introducing the particles into the carrier flow. The inventive particle injector is characterized in that the carrier flow channel has substantially no dead volume.

Particle injector for a cell sorter

A method is described for non-destructive measurement of vitality of biological cells, especially for determination of apoptosis, in which the at least one cell is exposed to high-frequency alternating, especially rotating, electric fields and/or impedance test fields, and at least one rotation measurement, one dielectrophoresis measurement and/or one impedance measurement is performed with the cell for at least one frequency range or individual frequencies, from which at least one measurement parameter is determined that is characteristic of the vitality state of the cell.

Method for measuring the vitality of cells

A coupling device (), particularly for liquid-tight coupling of at least one liquid line () to a fluidic system (), is described, which comprises: at least one sealing device (), which is implemented to receive an end region () of the liquid line () and has a first sealing surface () for placement on an external surface () of the fluidic system in such a way that the end of the liquid line () is laterally enclosed by the first sealing surface () and points toward an opening () in the external surface (), and a clamping device (), using which the sealing device () may be pressed against the fluidic system (), so that the first sealing surface () produces a liquid-tight connection with the external surface ().

Device and method for coupling lines to fluidic microsystems

Described is a fluidic microsystem () including at least one channel () through which a particle suspension can flow; and first and second electrode devices () which are arranged on first and second channel walls () for generating electrical alternating-voltage fields in the channel (); wherein the first electrode device () for field shaping in the channel includes at least one first structure element (); and the second electrode device () includes an area-like electrode layer () with a closed second electrode surface which includes a second passivation layer (); wherein the effective electrode surface of the first structure element (), of which element () there is at least one, is smaller than the second electrode surface; and the second passivation layer () is a closed layer which completely covers the second electrode layer ().

Fluidic microsystem comprising field-forming passivation layers provided on microelectrodes

A method of discharging a fluid flow with suspended microparticles from a fluidic microsystem () is described, whereby the fluid flow converges with at least one output flow to form a discharge flow at the end of a discharge channel () of the microsystem, and the discharge flow is delivered through a conduction element (). A microsystem with a flow output device for implementation of this method is also described.

Method and device for withdrawing suspended microparticles from a fluidic microsystem

The invention relates to a method for generating a convective liquid motion in a fluidic microsystem. According to this method, a liquid in a microsystem is simultaneously exposed to an electrical field and a thermal gradient. The electrical field is generated by means of an electrode arrangement which is subjected to a time-variant voltage. In this way, a time variant electrical field is formed in the liquid volume. The thermal gradient is produced by means of at least one radiation absorber located in the compartment which is exposed to at least one external radiation field.

Method and device for generating microconvections

Described is a fluidic microsystem with at least one compartment for the receiving and/or the through-flow of a liquid and having an electrode arrangement with a multiplicity of electrodes, between which a coaction zone is established, whereby the compartment possesses at least one wall, through which electromagnetic radiation can be linked into the said coaction zone in accord with a predetermined incident direction, and on at least one electrode a cooling apparatus is provided, and on which at least one respective electrode at least one reflector layer is provided, and wherein the respective electrode is at least partially shielded in reference to the incident beam direction, and having at least one heat conducting layer by means of which the respective electrode stands in thermal communication with a wall of the compartment, and/or includes an active cooling element, which is placed in thermal contact with the respective electrode.

Growing community of inventors

Berlin, Germany

Torsten Müller