Stmicroelectronics S.r.l.

Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.v.

Perkinelmer Cellular Technologies Germany Gmbh

Torsten Mueller

Thomas Schnelle

Rolf Hagedorn

Guenter R Fuhr

Bernd Wagner

Andreas Voigt

Mario Giovanni Scurati

Stefan Fiedler

Henning Glasser

Jan Hornung

A plurality of planar electrodes () in a microchannel () is used for separation, lysis and PCR in a chip (). Cells from a sample are brought to the electrodes (). Depending on sample properties, phase pattern, frequency and voltage of the electrodes and flow velocity are chosen to trap target cells () using DEP, whereas the majority of unwanted cells () flushes through. After separation the target cell () are lysed while still trapped. Lysis is carried out by applying RF pulses and/or thermally so as to change the dielectric properties of the trapped cells. After lysis, the target cells () are amplified within the microchannel (), so as to obtain separation, lysis and PCR on same chip ().

Treatment of biological samples using dielectrophoresis

Shaping of microparticles in electric-field cages

Growing community of inventors

Berlin, Germany

Torsten Mueller